Modified solid lipid nanoparticles encapsulated with Amphotericin B and Paromomycin: an effective oral combination against experimental murine visceral leishmaniasis.

Scientific Reports
Shabi ParvezShyam Lal Mudavath

Abstract

The development of an effective oral therapeutics is an immediate need for the control and elimination of visceral leishmaniasis (VL). We exemplify the preparation and optimization of 2-hydroxypropyl-β-cyclodextrin (HPCD) modified solid lipid nanoparticles (SLNs) based oral combinational cargo system of Amphotericin B (AmB) and Paromomycin (PM) against murine VL. The emulsion solvent evaporation method was employed to prepare HPCD modified dual drug-loaded solid lipid nanoparticles (m-DDSLNs). The optimized formulations have a mean particle size of 141 ± 3.2 nm, a polydispersity index of 0.248 ± 0.11 and entrapment efficiency for AmB and PM was found to be 96% and 90% respectively. The morphology of m-DDSLNs was confirmed by scanning electron microscopy and transmission electron microscopy. The developed formulations revealed a sustained drug release profile upto 57% (AmB) and 21.5% (PM) within 72 h and were stable at both 4 °C and 25 °C during short term stability studies performed for 2 months. Confocal laser scanning microscopy confirmed complete cellular internalization of SLNs within 24 h of incubation. In vitro cytotoxicity study against J774A.1 macrophage cells confirmed the safety and biocompatibility of the developed f...Continue Reading

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Citations

Apr 17, 2021·Journal of Nanobiotechnology·Humzah JamshaidGul Majid Khan
Jul 31, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Pragya PrasannaDebabrata Mandal
Aug 25, 2021·European Journal of Pharmacology·Diksha KumariKuljit Singh
Sep 24, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Garima JoshiKhushwant S Yadav

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Methods Mentioned

BETA
fluorescence spectroscopy
scanning electron microscopy
transmission
infrared spectroscopy
X-ray
electron microscopy
Confocal microscopy

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