Modified vaccinia virus Ankara exerts potent immune modulatory activities in a murine model.

PloS One
Miriam NörderCarlos A Guzman

Abstract

Modified vaccinia virus Ankara (MVA), a highly attenuated strain of vaccinia virus, has been used as vaccine delivery vector in preclinical and clinical studies against infectious diseases and malignancies. Here, we investigated whether an MVA which does not encode any antigen (Ag) could be exploited as adjuvant per se. We showed that dendritic cells infected in vitro with non-recombinant (nr) MVA expressed maturation and activation markers and were able to efficiently present exogenously pulsed Ag to T cells. In contrast to the dominant T helper (Th) 1 biased responses elicited against Ags produced by recombinant MVA vectors, the use of nrMVA as adjuvant for the co-administered soluble Ags resulted in a long lasting mixed Th1/Th2 responses. These findings open new ways to potentiate and modulate the immune responses to vaccine Ags depending on whether they are co-administered with MVA or encoded by recombinant viruses.

References

Dec 1, 1992·The Journal of Experimental Medicine·B CatipovićJ P Schneck
Jul 10, 1997·Nature·R A ManzA Radbruch
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J M RobertsonB D Evavold
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Dec 12, 2001·Journal of Virology·Valérie Molinier-FrenkelJean-Gérard Guillet
Mar 29, 2002·European Journal of Immunology·Rudolf A Manz, Andreas Radbruch
Apr 22, 2003·BioTechniques·Caroline StaibGerd Sutter
Oct 11, 2003·The Journal of Infectious Diseases·Vasee S MoorthyAdrian V S Hill
Dec 9, 2003·Trends in Molecular Medicine·Volker SchirrmacherPhilipp Beckhove
Apr 15, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C BarbeyF Spertini
Jul 23, 2004·The Journal of General Virology·Robert DrillienDaniel Hanau
Dec 21, 2004·Springer Seminars in Immunopathology·Kenneth L RockYan Shi
Mar 3, 2005·Medical Immunology·Mark K Slifka
Jun 18, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A P D SouzaA R Pinto
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claire L HutchingsAnne C Moore
Apr 18, 2006·Current Opinion in Immunology·Katrin MoserRudolf A Manz
Mar 16, 2007·European Journal of Immunology·Jens Y HumrichLars Jenne
Jun 15, 2007·Journal of the American Academy of Dermatology·Eric L SimpsonJon M Hanifin
Aug 22, 2007·The Journal of Experimental Medicine·Wolfgang KastenmullerIngo Drexler
Apr 10, 2008·The Journal of Clinical Investigation·Christofer SamuelssonHubertus Hochrein

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Citations

Feb 23, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Arturo Reyes-SandovalAdrian V S Hill
Nov 18, 2011·Cancer Immunology, Immunotherapy : CII·Debora MartorelliMaria G Masucci
Jan 1, 2020·Expert Review of Vaccines·Lasse NeukirchPeter Johannes Holst

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Methods Mentioned

BETA
dissection
flow cytometry
FACS
ELISA
glycosylation
Assay

Software Mentioned

ImmunoSpot image analyzer
GraphPad Prism
FACS Diva

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