Modify on the fly: triple quad to high resolution in support of a dermal clinical study requiring an ultra low LLOQ

Bioanalysis
Chester L BowenChristopher Evans

Abstract

FTIH studies can be challenging due to the varying dosing regimens and rapid data delivery. Chemists are asked to provide ultra-low limits of quantitation to provide an understanding of patient efficacy and safety in order to progress drug development. In a recent dermal study it became necessary to reduce the LLOQ of a small molecule drug from 50 to 1 pg/ml due to reductions in the dose and surface area of drug application. The 50-fold increase in assay sensitivity necessitated the use of a high-resolution mass spectrometer (LC-HRMS) to separate matrix interferences observed when using a unit resolution triple quadrupole MS. A sensitive, robust assay was validated to support of a FTIH study using a LC-HRMS.

References

Mar 6, 2009·Rapid Communications in Mass Spectrometry : RCM·Nanyan Rena ZhangWilliam Bart Emary
Apr 3, 2009·Journal of the American Society for Mass Spectrometry·Kevin P BatemanLester Taylor
Apr 25, 2009·Journal of the American Academy of Dermatology·Diane ThiboutotUNKNOWN Global Alliance to Improve Outcomes in Acne
Dec 7, 2010·Therapeutic Delivery·Kalpana S PaudelAudra L Stinchcomb

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