Modifying effects of vitamin e on chlorpyrifos toxicity in atlantic salmon

PloS One
Pål A OlsvikLiv Søfteland

Abstract

The aim of this study was to elucidate how vitamin E (alpha tocopherol) may ameliorate the toxicity of the pesticide chlorpyrifos in Atlantic salmon. Freshly isolated hepatocytes were exposed to vitamin E, chlorpyrifos or a combination of vitamin E and chlorpyrifos (all 100 μM). Transcriptomics (RNA-seq) and metabolomics were used to screen for effects of vitamin E and chlorpyrifos. By introducing vitamin E, the number of upregulated transcripts induced by chlorpyrifos exposure was reduced from 941 to 626, while the number of downregulated transcripts was reduced from 901 to 742 compared to the control. Adding only vitamin E had no effect on the transcriptome. Jak-STAT signaling was the most significantly affected pathway by chlorpyrifos treatment according to the transcriptomics data. The metabolomics data showed that accumulation of multiple long chain fatty acids and dipeptides and amino acids in chlorpyrifos treated cells was partially alleviated by vitamin E treatment. Significant interaction effects between chlorpyrifos and vitamin E were seen for 15 metabolites, including 12 dipeptides. The antioxidant had relatively modest effects on chlorpyrifos-induced oxidative stress. By combining the two data sets, the study sugges...Continue Reading

References

Sep 25, 1999·Archives of Biochemistry and Biophysics·B S KristalW R Matson
Nov 18, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Young Jin MoonMarilyn E Morris
Aug 21, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Anna Lukaszewicz-Hussain
Mar 7, 2008·Pest Management Science·Ernest Hodgson, Randy L Rose
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jul 18, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Liv SøftelandPål A Olsvik
Aug 26, 2009·Current Drug Metabolism·María José Gómez-LechónMaría Teresa Donato
Oct 31, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Anne Vatland KrøvelPål A Olsvik
Nov 28, 2009·Free Radical Biology & Medicine·Eric E KelleyMargaret M Tarpey
Mar 31, 2010·Toxicology and Applied Pharmacology·Estefania G MoreiraElaine M Faustman
Oct 5, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Suleiman Folorunsho AmbaliKing A Nelson Esievo
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Oct 5, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Pål A OlsvikBente E Torstensen
Feb 9, 2012·Bulletin of Environmental Contamination and Toxicology·Elif Oruc
Aug 18, 2012·Journal of Aquatic Animal Health·WeiNa XuXianngFei Li
Dec 1, 2012·International Journal of Toxicology·J Phillip KaiserScott C Wesselkamper
Jan 17, 2013·CA: a Cancer Journal for Clinicians·Michael C R AlavanjaMatthew R Bonner
Jun 1, 2013·CA: a Cancer Journal for Clinicians·Michael C R AlavanjaMatthew R Bonner
Jun 1, 2013·CA: a Cancer Journal for Clinicians·James W GrayWilliam M Mahlburg
Jun 6, 2013·Biochimica Et Biophysica Acta·Allison J Richard, Jacqueline M Stephens
Sep 7, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Liv SøftelandPål A Olsvik

❮ Previous
Next ❯

Citations

Sep 15, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Pål A OlsvikLiv Søfteland
Mar 8, 2017·Chemical Research in Toxicology·Dan CarmanyJennifer W Sekowski
Jan 10, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K DaleS Ellingsen
Apr 14, 2020·PloS One·Edisson Duarte-RestrepoLaura Duarte-Jaramillo
Aug 4, 2016·Journal of Toxicology and Environmental Health. Part a·Pål A OlsvikTorstein Kristensen
Nov 18, 2017·Journal of Applied Toxicology : JAT·Pål A Olsvik, Liv Søfteland
Nov 13, 2018·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·P A OlsvikM Sanden
Mar 23, 2021·The Science of the Total Environment·Donna A GlinskiW Matthew Henderson

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
RNA-seq

Software Mentioned

Core
IPA
R
DESeq
RTCA
IPA Core
BLAST2GO
Blastx
IPA Compare

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
M Kitagawa, M Mino
The Journal of Obstetrics and Gynaecology of the British Empire
E SHUTE
Journal of Cell Science
Jason S RawlingsDouglas A Harrison
© 2021 Meta ULC. All rights reserved