Modular 31 P wideband inversion transfer for integrative analysis of adenosine triphosphate metabolism, T1 relaxation and molecular dynamics in skeletal muscle at 7T

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Jimin RenCraig R Malloy

Abstract

For efficient and integrative analysis of de novo adenosine triphosphate (ATP) synthesis, creatine-kinase-mediated ATP synthesis, T1 relaxation time, and ATP molecular motion dynamics in human skeletal muscle at rest. Four inversion-transfer modules differing in center inversion frequency were combined to generate amplified magnetization transfer (MT) effects in targeted MT pathways, including Pi ↔ γ-ATP, PCr ↔ γ-ATP, and 31 Pγ(α)ATP ↔ 31 PβATP . MT effects from both forward and reverse exchange kinetic pathways were acquired to reduce potential bias and confounding factors in integrated data analysis. Kinetic data collected using 4 wideband inversion modules (8 minutes each) yielded the forward exchange rate constants, kPCr→γATP = 0.31 ± 0.05 s-1 and kPi→γATP = 0.064 ± 0.012 s-1 , and the reverse exchange rate constants, kγATP→Pi = 0.034 ± 0.006 s-1 and kγATP→PCr = 1.37 ± 0.22 s-1 , respectively. The cross-relaxation rate constant, σγ(α) ↔ βATP was -0.20 ± 0.03 s-1 , corresponding to ATP rotational correlation time τc of 0.8 ± 0.1 × 10-7 seconds. The intrinsic T1 relaxation times were Pi (9.2 ± 1.4 seconds), PCr (6.2 ± 0.4 seconds), γ-ATP (1.8 ± 0.1 seconds), α-ATP (1.4 ± 0.1 seconds), and β-ATP (1.1 ± 0.1 seconds). Muscle ATP...Continue Reading

References

Apr 30, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Paul A BottomleyRobert G Weiss
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marcus E Raichle, Debra A Gusnard
May 17, 2003·Science·Kitt Falk PetersenGerald I Shulman
Dec 1, 2004·Acta Physiologica Scandinavica·D J Marcinek
Dec 20, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Vera B Schrauwen-HinderlingPatrick Schrauwen
Jan 30, 2008·Journal of Magnetic Resonance·Refaat E GabrPaul A Bottomley
Apr 23, 2008·European Journal of Endocrinology·H M De FeyterJ J Prompers
Feb 6, 2009·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·AbdEl-Monem El-SharkawyPaul A Bottomley
Jun 16, 2009·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·W BognerS Gruber
Dec 29, 2010·Journal of Nutrition and Metabolism·Julien S BakerRobert A Robergs
May 11, 2011·The Journal of Clinical Investigation·Alison SleighDavid B Savage
Dec 14, 2011·European Journal of Radiology·Ladislav ValkovičWolfgang Bogner
Feb 8, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Prodromos ParasoglouRavinder R Regatte
Jan 29, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Ladislav ValkovičMarek Chmelík
Feb 27, 2014·The International Journal of Biochemistry & Cell Biology·Jeanine J PrompersKlaas Nicolay
Mar 13, 2014·Muscle & Nerve·Terence E RyanKevin K Mccully
Apr 16, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jimin RenCraig R Malloy
Dec 4, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jimin RenCraig R Malloy
Apr 12, 2016·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jimin RenCraig R Malloy
Jul 28, 2016·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Bertrand PouymayouChris Boesch
Aug 18, 2016·Frontiers in Physiology·Anna BogdanovaAntonio Martínez-Ruiz
Nov 22, 2016·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jimin RenCraig R Malloy
Mar 14, 2017·Journal of Muscle Research and Cell Motility·C J Barclay

❮ Previous
Next ❯

Citations

Feb 11, 2020·NMR in Biomedicine·Martin MeyerspeerUNKNOWN Experts' Working Group on 31P MR Spectroscopy of Skeletal Muscle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.