Modular design of metabolic network for robust production of n-butanol from galactose-glucose mixtures

Biotechnology for Biofuels
Hyun Gyu LimGyoo Yeol Jung

Abstract

Refactoring microorganisms for efficient production of advanced biofuel such as n-butanol from a mixture of sugars in the cheap feedstock is a prerequisite to achieve economic feasibility in biorefinery. However, production of biofuel from inedible and cheap feedstock is highly challenging due to the slower utilization of biomass-driven sugars, arising from complex assimilation pathway, difficulties in amplification of biosynthetic pathways for heterologous metabolite, and redox imbalance caused by consuming intracellular reducing power to produce quite reduced biofuel. Even with these problems, the microorganisms should show robust production of biofuel to obtain industrial feasibility. Thus, refactoring microorganisms for efficient conversion is highly desirable in biofuel production. In this study, we engineered robust Escherichia coli to accomplish high production of n-butanol from galactose-glucose mixtures via the design of modular pathway, an efficient and systematic way, to reconstruct the entire metabolic pathway with many target genes. Three modular pathways designed using the predictable genetic elements were assembled for efficient galactose utilization, n-butanol production, and redox re-balancing to robustly produ...Continue Reading

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Citations

Jan 28, 2017·Biotechnology for Biofuels·Dong Hun KwakGyoo Yeol Jung
Mar 3, 2018·Applied Microbiology and Biotechnology·Nan XuLiming Liu
Jun 27, 2017·Biotechnology for Biofuels·Jae Hyung Lim, Gyoo Yeol Jung
Jun 7, 2019·Nature Communications·Hyun Gyu LimGyoo Yeol Jung
Nov 11, 2016·Journal of Industrial Microbiology & Biotechnology·Byung Eun MinGyoo Yeol Jung
Jul 3, 2021·International Journal of Molecular Sciences·Da-Ae GwonJeong Wook Lee
Apr 12, 2018·Journal of Agricultural and Food Chemistry·Hyun Gyu LimGyoo Yeol Jung

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Methods Mentioned

BETA
PCR

Software Mentioned

UTR Designer

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