Abstract
Microfluidic systems are very useful for in vitro studies of interactions between blood cells and vascular endothelial cells under flow, and several commercial solutions exist. However, the availability of customizable, user-designed devices is largely restricted to researchers with expertise in photolithography and access to clean room facilities. Here we describe a strategy for producing tailor-made modular microfluidic systems, cast in PDMS from 3D-printed molds, to facilitate studies of leukocyte adherence to endothelial cells. A dual-chamber barrier module was optimized for culturing two endothelial cell populations, separated by a 250 μm wide dividing wall, on a glass slide. In proof-of-principle experiments one endothelial population was activated by TNFα, while the other served as an internal control. The barrier module was thereafter replaced with a microfluidic flow module, enclosing both endothelial populations in a common channel. A suspension of fluorescently-labeled leukocytes was then perfused through the flow module and leukocyte interactions with control and TNFα-treated endothelial populations were monitored in the same field of view. Time-lapse microscopy analysis confirmed the preferential attachment of leuk...Continue Reading
References
May 26, 2004·Immunology Letters·Toshiyuki MuraiMasayuki Miyasaka
Dec 24, 2004·Lab on a Chip·Wesley C ChangDorian Liepmann
Jul 28, 2006·Nature·George M Whitesides
Oct 13, 2006·Annals of Biomedical Engineering·Lakeshia J TaiteJennifer L West
Mar 29, 2007·Lab on a Chip·Ulrich Y SchaffScott I Simon
Nov 22, 2008·Lab on a Chip·He ZhuAlexander Revzin
Feb 5, 2009·Lab on a Chip·Irmeli BarkeforsJohan Kreuger
Dec 4, 2012·Journal of Biomechanics·Elena BianchiGabriele Dubini
Nov 28, 2013·Lab on a Chip·Germán CominaDaniel Filippini
Jan 18, 2014·Analytical Chemistry·Bethany C GrossDana M Spence
Feb 11, 2014·Lab on a Chip·Anthony K AuAlbert Folch
Mar 14, 2014·Nature·Eric K SackmannDavid J Beebe
Apr 26, 2014·Lab on a Chip·Jayda L ErkalDana M Spence
Aug 20, 2014·Analytical Chemistry·Giuseppina LambertiMohammad F Kiani
Nov 2, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hua HuangAnna Dimberg
Mar 21, 2015·PLoS Biology·Tom BadenThomas Euler
Jul 24, 2015·Scientific Reports·Robert G ManninoWilbur A Lam
Sep 10, 2015·Journal of Cell Science·Jaeho OhSusan N Thomas
Oct 28, 2015·Scientific Reports·Hansang ChoDaniel Irimia
May 18, 2016·Biomicrofluidics·Eddie A ShimpDavid W Schmidtke
Jun 21, 2016·Biofabrication·Reza AminSavas Tasoglu
Dec 22, 2016·PloS One·Rodrigo Hernández VeraJohan Kreuger
Feb 15, 2017·Analytical Chemistry·Miao SunJan C T Eijkel
Mar 25, 2017·Scientific Reports·Daria TsvirkunLionel Bureau
Jul 10, 2018·Biomicrofluidics·Barbara BachmannPeter Ertl
Jul 22, 2018·Scientific Reports·Ayoola BrimmoMohammad A Qasaimeh
Feb 21, 2019·Lab on a Chip·Nikos Fatsis-KavalopoulosJohan Kreuger
Jul 3, 2018·International Journal of Bioprinting·Denise MandtAleksandr Ovsianikov
Citations
Apr 3, 2021·Drug Delivery and Translational Research·Justin SilbermanErika Moore
Apr 16, 2021·Biomedical Microdevices·James J TronoloneKyung Sung
Jun 3, 2021·International Journal of Molecular Sciences·Katharina UrschelBarbara Dietel
Jul 25, 2021·Cancers·Alessandro PoggiRoberto Benelli
Dec 3, 2020·Analytical Chemistry·Hari Kalathil BalakrishnanRosanne M Guijt