Modular nanotransporters: a multipurpose in vivo working platform for targeted drug delivery.

International Journal of Nanomedicine
Tatiana A SlastnikovaAlexander S Sobolev

Abstract

Modular nanotransporters (MNT) are recombinant multifunctional polypeptides created to exploit a cascade of cellular processes, initiated with membrane receptor recognition to deliver selective short-range and highly cytotoxic therapeutics to the cell nucleus. This research was designed for in vivo concept testing for this drug delivery platform using two modular nanotransporters, one targeted to the α-melanocyte-stimulating hormone (αMSH) receptor overexpressed on melanoma cells and the other to the epidermal growth factor (EGF) receptor overexpressed on several cancers, including glioblastoma, and head-and-neck and breast carcinoma cells. In vivo targeting of the modular nanotransporter was determined by immuno-fluorescence confocal laser scanning microscopy and by accumulation of (125)I-labeled modular nanotransporters. The in vivo therapeutic effects of the modular nanotransporters were assessed by photodynamic therapy studies, given that the cytotoxicity of photosensitizers is critically dependent on their delivery to the cell nucleus. Immunohistochemical analyses of tumor and neighboring normal tissues of mice injected with multifunctional nanotransporters demonstrated preferential uptake in tumor tissue, particularly in ...Continue Reading

References

Aug 15, 1997·The Journal of Biological Chemistry·T V AkhlyninaA S Sobolev
May 14, 1998·The Journal of Investigative Dermatology·K W WoodburnS W Young
Aug 12, 1999·European Journal of Pharmacology·J E Wikberg
Apr 27, 2000·Progress in Biophysics and Molecular Biology·A S SobolevA A Rosenkranz
Jun 17, 2000·The International Journal of Biochemistry & Cell Biology·B CunderlíkováJ Moan
May 10, 2001·Current Opinion in Cell Biology·E Conti, E Izaurralde
Feb 21, 2002·Molecular Biology of the Cell·Nelly Panté, Michael Kann
May 29, 2002·The EMBO Journal·Katharina Ribbeck, Dirk Görlich
Apr 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrey A RosenkranzAlexander S Sobolev
May 2, 2003·Nature Reviews. Cancer·Dennis E J G J DolmansRakesh K Jain
Aug 16, 2003·Endocrine Reviews·Jean Claude Reubi
Aug 4, 2004·The Lancet Oncology·Stanley B BrownIan Walker
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Omid C FarokhzadRobert Langer
Nov 3, 2006·Cancer Research·Dinara G GilyazovaAlexander S Sobolev
Apr 5, 2007·Nature Protocols·Ganesan Vaidyanathan, Michael R Zalutsky
Jul 17, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Danny L CostantiniRaymond M Reilly
Sep 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Derek W BartlettMark E Davis
Sep 21, 2007·Photochemistry and Photobiology·Sarika VermaTayyaba Hasan
Nov 6, 2007·Journal of Photochemistry and Photobiology. B, Biology·Clara FabrisMarina Soncin
Feb 23, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexander S Sobolev
Apr 9, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·William Wei Lim ChinMalini Olivo
Apr 30, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yuri V KhramtsovAlexander S Sobolev
Aug 30, 2008·International Journal of Radiation Oncology, Biology, Physics·Andrey A RosenkranzAlexander S Sobolev
Sep 2, 2008·Nature Reviews. Drug Discovery·Mark E DavisDong M Shin
Apr 21, 2009·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·G JoriC Fabris
Jun 6, 2009·Cellular and Molecular Life Sciences : CMLS·Hervé Hillaireau, Patrick Couvreur
Sep 10, 2009·Clinical and Experimental Immunology·E MartinelliF Ciardiello
Oct 15, 2009·Molecular Cancer Therapeutics·Michael M Schmidt, K Dane Wittrup
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chung Hang J ChoiMark E Davis
Sep 15, 2010·Nature Reviews. Clinical Oncology·Rakesh K Jain, Triantafyllos Stylianopoulos
Sep 30, 2010·ACS Nano·James H AdairMark Kester
Nov 3, 2010·International Journal of Nanomedicine·Michelle Pautler, Sara Brenner

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Citations

Jul 26, 2012·Journal of Signal Transduction·Valentina CeaChiara Verpelli
Dec 3, 2014·Cancer Letters·M C F SimõesA A C C Pais
Aug 29, 2020·International Journal of Radiation Biology·Andrey A RosenkranzAlexander S Sobolev
Feb 7, 2019·Frontiers in Pharmacology·David A Jans, Alexander S Sobolev
Sep 19, 2020·Antioxidants·Eugene G MaksimovNikolai N Sluchanko
Sep 14, 2018·Frontiers in Pharmacology·Alexander S Sobolev
Oct 15, 2020·Biochemistry. Biokhimii︠a︡·A A Rosenkranz, T A Slastnikova

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Methods Mentioned

BETA
gel filtration
xenografts
nuclear translocation
xenograft
enzyme-linked immunosorbent assay

Software Mentioned

SigmaPlot

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