Modular Optimization of a Hemicellulose-Utilizing Pathway in Corynebacterium glutamicum for Consolidated Bioprocessing of Hemicellulosic Biomass

ACS Synthetic Biology
Sung Sun YimKi Jun Jeong

Abstract

Hemicellulose, which is the second most abundant polysaccharide in nature after cellulose, has the potential to become a major feedstock for microbial fermentation to produce various biofuels and chemicals. To utilize hemicellulose economically, it is necessary to develop a consolidated bioprocess (CBP), in which all processes from biomass degradation to the production of target products occur in a single bioreactor. Here, we report a modularly engineered Corynebacterium glutamicum strain suitable for CBP using hemicellulosic biomass (xylan) as a feedstock. The hemicellulose-utilizing pathway was divided into three distinct modules, and each module was separately optimized. In the module for xylose utilization, the expression level of the xylose isomerase (xylA) and xylulokinase (xylB) genes was optimized with synthetic promoters of different strengths. Then, the module for xylose transport was engineered with combinatorial sets of synthetic promoters and heterologous transporters to achieve the fastest cell growth rate on xylose (0.372 h(-1)). Next, the module for the enzymatic degradation of xylan to xylose was also engineered with different combinations of promoters and signal peptides to efficiently secrete both endoxylanas...Continue Reading

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Citations

Mar 21, 2018·Applied Microbiology and Biotechnology·Kei-Anne BaritugoSi Jae Park
Jul 10, 2018·FEMS Microbiology Letters·Fernando Pérez-García, Volker F Wendisch
Sep 16, 2018·Applied Microbiology and Biotechnology·Nannan ZhaoSuiping Zheng
Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Bin ZhangXiao-Yu Wu

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