PMID: 9192618Jun 24, 1997Paper

Modular organization of the catalytic center of RNA polymerase

Proceedings of the National Academy of Sciences of the United States of America
A MustaevA Goldfarb

Abstract

The Fe2+ ion that specifically replaces Mg2+ in the active center of RNA polymerase generates reactive hydroxyl radicals that cause highly localized cleavage of polypeptide chains. Mapping of the cleavage sites revealed the overall architecture of the active center. Nine distinct sites, five in the beta subunit and four in the beta' subunit of Escherichia coli RNA polymerase, all at or near highly conserved sequence motifs, are brought together in the enzyme's ternary structure within the distance of approximately 1 nm from the active center Me2+. These sites are located in at least six different domains of the subunits, reflecting modular organization of the active center.

References

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Citations

Jan 20, 2009·Applied Microbiology and Biotechnology·Vincent RenoufAline Lonvaud-Funel
Oct 7, 2006·The Protein Journal·Jolanta SereikaiteGervydas Dienys
Apr 12, 2001·Current Opinion in Structural Biology·S A Darst
Dec 8, 2009·Nature Structural & Molecular Biology·Jinwei ZhangRobert Landick
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Frédéric CollandAnnie Kolb
Sep 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Andrey FeklistovRichard H Ebright
Oct 27, 2009·The Journal of Biological Chemistry·Ekaterina StepanovaSergei Borukhov
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S A DarstG Zhang
Jul 28, 2011·Molekuliarnaia biologiia·S A Proshkin, A S Mironov
Jun 6, 2009·Annual Review of Biochemistry·Evgeny Nudler
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J T OwensC F Meares
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J W StillerB D Hall
Oct 23, 2015·Nucleic Acids Research·Maria YakuninaLeonid Minakhin
Jun 5, 2015·Scientific Reports·Johanna DrögemüllerPaul Rösch
Nov 13, 2015·Scientific Reports·Johanna DrögemüllerStefan H Knauer
Dec 8, 2014·Journal of Biotechnology·Pietro AlifanoAdelfia Talà
Mar 14, 2001·Biochimica Et Biophysica Acta·C L Hawkins, M J Davies
Aug 2, 2013·The Journal of Biological Chemistry·Meng-Lun HsiehDeborah M Hinton
Jun 18, 2003·The Journal of General Virology·Jane S TitteringtonA Lorena Passarelli
Aug 19, 2007·The Journal of Biological Chemistry·Anoop CheeranRanjan Sen
Mar 31, 2016·The Biochemical Journal·Michael J Davies
Dec 14, 2002·The Journal of Biological Chemistry·Airat Valiakhmetov, David S Perlin
Feb 1, 2000·The Journal of Biological Chemistry·A KatayamaA Ishihama
Jul 12, 2013·The Journal of Biological Chemistry·Paramita SarkarDipankar Chatterji
Feb 1, 2000·The Journal of Biological Chemistry·K BrodolinV Nikiforov
Nov 27, 2020·Biomolecules·Unnatiben Rajeshbhai PatelDipankar Chatterji

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