Modular Organization of the NusA- and NusG-Stimulated RNA Polymerase Pause Signal That Participates in the Bacillus subtilis trp Operon Attenuation Mechanism

Journal of Bacteriology
Smarajit MondalPaul Babitzke

Abstract

The Bacillus subtilis trpEDCFBA operon is regulated by a transcription attenuation mechanism in which tryptophan-activated TRAP binds to the nascent transcript and blocks the formation of an antiterminator structure such that the formation of an overlapping intrinsic terminator causes termination in the 5' untranslated region (5' UTR). In the absence of bound TRAP, the antiterminator forms and transcription continues into the trp genes. RNA polymerase pauses at positions U107 and U144 in the 5' UTR. The general transcription elongation factors NusA and NusG stimulate pausing at both positions. NusG-stimulated pausing at U144 requires sequence-specific contacts with a T tract in the nontemplate DNA (ntDNA) strand within the paused transcription bubble. Pausing at U144 participates in a trpE translation repression mechanism. Since U107 just precedes the critical overlap between the antiterminator and terminator structures, pausing at this position is thought to participate in attenuation. Here we carried out in vitro pausing and termination experiments to identify components of the U107 pause signal and to determine whether pausing affects the termination efficiency in the 5' UTR. We determined that the U107 and U144 pause signal...Continue Reading

References

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Citations

Aug 18, 2017·RNA Biology·Ivan PetushkovAndrey Kulbachinskiy
May 15, 2018·Molecular Microbiology·Vladimir Svetlov, Evgeny Nudler
May 19, 2019·Annual Review of Microbiology·Paul BabitzkeTony Romeo
Jul 5, 2019·Microbiology and Molecular Biology Reviews : MMBR·Charles L Turnbough
Oct 3, 2020·Critical Reviews in Biochemistry and Molecular Biology·Alexander V YakhninPaul Babitzke

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