Modular self-assembly of gamma-modified peptide nucleic acids in organic solvent mixtures.

Nature Communications
Sriram KumarRebecca E Taylor

Abstract

Nucleic acid-based materials enable sub-nanometer precision in self-assembly for fields including biophysics, diagnostics, therapeutics, photonics, and nanofabrication. However, structural DNA nanotechnology has been limited to substantially hydrated media. Transfer to organic solvents commonly used in polymer and peptide synthesis results in the alteration of DNA helical structure or reduced thermal stabilities. Here we demonstrate that gamma-modified peptide nucleic acids (γPNA) can be used to enable formation of complex, self-assembling nanostructures in select polar aprotic organic solvent mixtures. However, unlike the diameter-monodisperse populations of nanofibers formed using analogous DNA approaches, γPNA structures appear to form bundles of nanofibers. A tight distribution of the nanofiber diameters could, however, be achieved in the presence of the surfactant SDS during self-assembly. We further demonstrate nanostructure morphology can be tuned by means of solvent solution and by strand substitution with DNA and unmodified PNA. This work thereby introduces a science of γPNA nanotechnology.

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J C Wang
Aug 11, 1978·Science·J D Griffith
May 1, 1996·Nature Structural Biology·M Eriksson, P E Nielsen
Sep 1, 1996·Nucleic Acids Research·P J FinnT Brown
Feb 1, 1997·Nature Structural Biology·H RasmussenP E Nielsen
May 1, 1995·Journal of Peptide Science : an Official Publication of the European Peptide Society·L ChristensenR H Berg
Apr 4, 2000·Biotechnology and Bioengineering·G Bonner, A M Klibanov
Feb 22, 2001·Methods : a Companion to Methods in Enzymology·D A Braasch, D R Corey
Oct 2, 2003·Nature Biotechnology·Shuguang Zhang
Nov 18, 2004·Bioconjugate Chemistry·James P VernilleJames W Schneider
Dec 17, 2004·Journal of the American Chemical Society·Paul W K RothemundErik Winfree
Mar 17, 2006·Nature·Paul W K Rothemund
Aug 3, 2006·Journal of the American Chemical Society·Anca Dragulescu-AndrasiDanith H Ly
May 5, 2007·Nucleic Acids Research·Anjana Sen, Peter E Nielsen
Dec 11, 2007·Nucleosides, Nucleotides & Nucleic Acids·Gian Maria BonoraLuigi Xodo
Jan 5, 1995·Biotechnology and Bioengineering·Z YangA J Russell
Aug 9, 2008·Science·Peng YinJohn H Reif
May 22, 2009·Nature·Shawn M DouglasWilliam M Shih
Aug 8, 2009·Science·Hendrik DietzWilliam M Shih
Nov 10, 2009·Nature Nanotechnology·Hareem T MauneErik Winfree
Mar 2, 2011·Nature Methods·Carlos Ernesto CastroHendrik Dietz
Aug 24, 2011·Journal of the American Chemical Society·Arivazhagan RajendranHiroshi Sugiyama
Dec 15, 2012·Science·Jean-Philippe J SobczakHendrik Dietz
Mar 28, 2013·Nano Letters·Yang YangHao Yan
Aug 1, 2014·Journal of the American Chemical Society·Carrie R JamesNathan C Gianneschi
Sep 23, 2014·Environmental Health and Toxicology·Xiaofang WangAhjeong Son
Apr 9, 2015·Soft Matter·Brad H JonesErik D Spoerke
Mar 24, 2016·Nature Chemistry·Nicole AvakyanHanadi F Sleiman
Jan 5, 2017·ACS Nano·Cheng TianHaitao Liu
Jan 27, 2017·ACS Nano·Alexander Mario MaierTim Liedl
May 12, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Yueying CaoJiqian Wang
Dec 17, 2017·Molecular Therapy. Nucleic Acids·Anisha GuptaPeter M Glazer
Feb 10, 2018·Science Advances·Boxuan ShenJ Jussi Toppari

❮ Previous
Next ❯

Citations

Dec 31, 2020·Pharmaceuticals·Stefano VolpiRoberto Corradini
Jul 3, 2021·Biopolymers·Sriram KumarRebecca E Taylor

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
nucleic

Software Mentioned

Advanced Techniques
Minitab
ImageJ
Fiji
Nikon NIS - Elements
Excel
Varian Cary

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.