Modulated Expression of the Protein Kinase GSK3 in Motor and Dopaminergic Neurons Increases Female Lifespan in Drosophila melanogaster

Frontiers in Genetics
Mikhail V TrostnikovElena G Pasyukova

Abstract

Most eukaryotic genes express multiple transcripts and proteins, and a sophisticated gene expression strategy plays a crucial role in ensuring the cell-specificity of genetic information and the correctness of phenotypes. The Drosophila melanogaster gene shaggy encodes several isoforms of the conserved glycogen synthase kinase 3 (GSK3), which is vitally important for multiple biological processes. To characterize the phenotypic effects of differential shaggy expression, we explored how the multidirectional modulation of the expression of the main GSK3 isoform, Shaggy-PB, in different tissues and cells affects lifespan. To this end, we used lines with transgenic constructs that encode mutant variants of the protein. The effect of shaggy misexpression on lifespan depended on the direction of the presumed change in GSK3 activity and the type of tissue/cell. The modulation of GSK3 activity in motor and dopaminergic neurons improved female lifespan but caused seemingly negative changes in the structural (mitochondrial depletion; neuronal loss) and functional (perturbed locomotion) properties of the nervous system, indicating the importance of analyzing the relationship between lifespan and healthspan in invertebrate models. Our find...Continue Reading

References

Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·L LiL J Houenou
Aug 1, 1993·Genetical Research·P R HoldenJ F Brookfield
Sep 6, 2000·Proceedings. Biological Sciences·J H Werren, D M Windsor
Nov 15, 2001·The Journal of Biological Chemistry·Elizabeth FraserTrevor C Dale
Sep 27, 2002·Genesis : the Journal of Genetics and Development·Marc Bourouis
Dec 10, 2003·Neuroreport·Gautam N Bijur, Richard S Jope
Mar 24, 2004·Current Opinion in Microbiology·Elizabeth A McGraw, Scott L O'Neill
May 15, 2004·Molecular and Cellular Biology·Deppie PapadopoulouMarc Bourouis
Jun 10, 2004·Current Biology : CB·Pankaj KapahiSeymour Benzer
Jun 12, 2004·Science·Maria E GiannakouLinda Partridge
Jul 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bénédicte FrancoYves Grau
Oct 20, 2004·Mechanisms of Ageing and Development·Chenxi WangDavid B Allison
Dec 28, 2004·Ageing Research Reviews·Angela L Ridgel, Roy E Ritzmann
Feb 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susan J BroughtonLinda Partridge
Jul 29, 2005·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·J Tonkiss, S K Calderwood
Nov 10, 2006·American Journal of Physiology. Cell Physiology·Oliver Kann, Richard Kovács
Apr 4, 2007·The Journal of Biological Chemistry·Abhinav K Jain, Anil K Jaiswal
Oct 2, 2007·Molecular and Cellular Neurosciences·Sigeng ChenDavid B Edelman
Jan 16, 2008·Developmental Cell·Gerasimos P Sykiotis, Dirk Bohmann
Mar 18, 2008·Free Radical Biology & Medicine·Derek A Drechsel, Manisha Patel
Jul 31, 2008·PloS One·Sigeng ChenDavid B Edelman
May 8, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Pamela F ColosimoNicholas S Tolwinski
Jul 22, 2010·Nature Reviews. Neuroscience·Eun-Mi Hur, Feng-Quan Zhou
Oct 26, 2010·Experimental Gerontology·Thomas Flatt
Jun 2, 2011·International Journal of Alzheimer's Disease·Calum Sutherland
Oct 7, 2011·Journal of Signal Transduction·Hang CuiHong Zhang
Nov 24, 2011·Frontiers in Molecular Neuroscience·Oksana Kaidanovich-Beilin, James Robert Woodgett
Mar 6, 2013·Antioxidants & Redox Signaling·William C OrrRajindar S Sohal

❮ Previous
Next ❯

Citations

Jun 15, 2021·Disease Models & Mechanisms·Stephanie M PützThomas Raabe

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
Assay

Software Mentioned

Tibco
LSM Image Browser
CFX Manager
Statistica
OASIS
ImageJ
Image Lab

Related Concepts

Related Feeds

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.