Modulated splicing-associated gene expression in P19 cells expressing distinct acetylcholinesterase splice variants

Journal of Neurochemistry
Shani Ben-AriY Ben-Shaul

Abstract

Alternative splicing configurations and acetylcholinesterase (AChE) gene expression are both modified in neurons under stress. However, it is unclear if these phenomena are functionally interrelated. Using a home-made spotted microarray focused on splicing-associated transcripts, we tested the effects of excess 3' splice variants of human AChE on splicing-related gene expression in semi-differentiated neuronal P19 cells. Of the tested transcripts, 17.3% and 20.2% showed modified expression levels (log2 of the ratio<-0.3 or>0.3) in transfected P19 cells overexpressing the stress-inducible AChE-R variant or the synaptic AChE-S protein, respectively. Multiple transcripts encoding serine-arginine rich (SR) and SR-related splicing regulators were suppressed in cells expressing either of these variants, whereas the gene groups including splicing-related helicases and transcripts involved in apoptosis displayed variant-specific changes. Our findings are compatible with the assumption that both neuronal overexpression and alternative splicing of pre-AChE mRNA may be causally involved in initiating global changes in neuronal alternative splicing, causing subsequent modifications in the expression patterns of numerous target genes.

References

Aug 1, 1982·The Journal of Cell Biology·E M Jones-VilleneuveV I Kalnins
Nov 1, 1995·The Journal of Experimental Medicine·D P Hughes, I N Crispe
Oct 28, 1998·Journal of Physiology, Paris·R M NitschH von der Kammer
Apr 2, 1999·The European Journal of Neuroscience·R DaoudS Stamm
Apr 3, 2001·Nature Reviews. Neuroscience·H Soreq, S Seidman
Jul 11, 2002·Cell Death and Differentiation·X J ZhangY F Shi
Sep 13, 2002·Nature·Zhaolan ZhouRobin Reed
Nov 28, 2002·Nature Genetics·Gary A Churchill
Jan 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Klara R BirikhHermona Soreq
Jul 31, 2003·Journal of Neurochemistry·Guang BaiPeter W Hoffman
Dec 20, 2003·Nature Reviews. Neuroscience·Mahmood Amiry-Moghaddam, Ole P Ottersen
Feb 28, 2004·FEBS Letters·Alan A DombkowskiRaymond F Novak
Apr 17, 2004·Cancer Research·Sang Eun ParkYoung Hyun Yoo
May 5, 2004·The Journal of Biological Chemistry·Eran MeshorerHermona Soreq
May 22, 2004·Neoplasia : an International Journal for Oncology Research·Chava PerryHermona Soreq
Sep 1, 2004·Cellular and Molecular Life Sciences : CMLS·C AkgulS W Edwards
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Chanseok Shin, James L Manley
Jan 20, 2005·Gene·Stefan StammHermona Soreq
Jun 1, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eran MeshorerYoram Cohen
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aljoscha NernS Lawrence Zipursky
Sep 13, 2005·Brain Research. Developmental Brain Research·Ki Jun KimPamela Pierce Palmer

❮ Previous
Next ❯

Citations

Aug 28, 2009·Journal of Molecular Neuroscience : MN·Ari MeersonDaniela Kaufer
Aug 3, 2007·Genome Research·Tony KwanJacek Majewski
Sep 5, 2008·PloS One·Debra ToiberHermona Soreq
Feb 14, 2012·Molecular Neurobiology·Giselli ScainiEmilio L Streck
Jan 27, 2009·Journal of Neuroscience Research·L Di Cesare MannelliC Ghelardini
Jan 22, 2008·The FEBS Journal·Hua Jiang, Xue-Jun Zhang
Jan 6, 2009·Annals of the New York Academy of Sciences·Iftach ShakedHermona Soreq
Aug 15, 2015·Thoracic Cancer·Hui-Jun XiZhao-Shen Li
Nov 19, 2011·Biochimica Et Biophysica Acta·Brian C GeyerHermona Soreq
Jan 17, 2015·Ecotoxicology and Environmental Safety·Kai WangJiazhen Jiang
Aug 8, 2015·Anais Da Academia Brasileira De Ciências·Brena P TeodorakEmilio L Streck

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.