Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins.

Scientific Reports
Anna M Duraj-ThatteNeel S Joshi

Abstract

Extracellular appendages play a significant role in mediating communication between bacteria and their host. Curli fibers are a class of bacterial fimbria that is highly amenable to engineering. We demonstrate the use of engineered curli fibers to rationally program interactions between bacteria and components of the mucosal epithelium. Commensal E. coli strains were engineered to produce recombinant curli fibers fused to the trefoil family of human cytokines. Biofilms formed from these strains bound more mucins than those producing wild-type curli fibers, and modulated mucin rheology as well. When treated with bacteria producing the curli-trefoil fusions mammalian cells behaved identically in terms of their migration behavior as when they were treated with the corresponding soluble trefoil factors. Overall, this demonstrates the potential utility of curli fibers as a scaffold for the display of bioactive domains and an untapped approach to rationally modulating host-microbe interactions using bacterial matrix proteins.

References

Dec 28, 1999·Gastroenterology·C TomasettoM C Rio
Nov 6, 2001·American Journal of Respiratory Cell and Molecular Biology·M OertelW Hoffmann
Feb 2, 2002·Science·Matthew R ChapmanScott J Hultgren
Aug 3, 2002·European Journal of Clinical Investigation·L ThimS S Poulsen
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Douglas Taupin, Daniel K Podolsky
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·W Hoffmann
May 18, 2006·Annual Review of Microbiology·Michelle M Barnhart, Matthew R Chapman
May 24, 2006·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Henri BraatLothar Steidler
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Jun 17, 2009·Cell Host & Microbe·Kimberly A KlineBirgitta Henriques-Normark
Nov 6, 2009·Journal of Medicinal Chemistry·Werner Hoffmann
Sep 8, 2010·Biochemical and Biophysical Research Communications·Guoyu YuYun Zhang
Nov 18, 2011·Trends in Microbiology·Nathalie Juge
Nov 13, 2012·Current Biology : CB·Marina CaldaraKatharina Ribbeck
May 31, 2013·Glycobiology·Angela MarcobalJustin L Sonnenburg
Jun 19, 2013·Proceedings of the National Academy of Sciences of the United States of America·Francesca TurroniMarco Ventura
Jan 15, 2014·Molecular Microbiology·Nani Van GervenHan Remaut
Apr 4, 2014·World Journal of Gastroenterology : WJG·Luise AamannHenning Grønbæk
Aug 1, 2014·ACS Synthetic Biology·Jan Claesen, Michael A Fischbach
Sep 18, 2014·Nature Communications·Peter Q NguyenNeel S Joshi
May 8, 2015·Biotechnology and Bioengineering·Zsofia BotyanszkiNeel S Joshi
Jun 13, 2015·Current Opinion in Biotechnology·Carlos Piñero-LambeaLuis Ángel Fernández
Jul 18, 2015·Microbiology Spectrum·Tyrrell Conway, Paul S Cohen
Sep 24, 2015·Nature Communications·Hai LiAndrew J Macpherson
Aug 9, 2016·Nature Reviews. Immunology·Malin E V Johansson, Gunnar C Hansson
Sep 19, 2016·Applied Microbiology and Biotechnology·Bingming OuGuoqiang Zhu
May 8, 2017·ACS Biomaterials Science & Engineering·Noémie-Manuelle Dorval CourchesneNeel S Joshi

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Citations

Apr 21, 2019·Applied and Environmental Microbiology·Anton KanNeel S Joshi
Dec 8, 2019·Nature Communications·Pichet PraveschotinuntNeel S Joshi
Jul 13, 2019·Frontiers in Aging Neuroscience·Carolina OsorioAdonis Sfera
Sep 11, 2019·International Journal of Molecular Sciences·Marguerite Clyne, Felicity E B May
Aug 14, 2019·Advanced Materials·Anna M Duraj-ThatteNeel S Joshi
Dec 24, 2018·Microbial Cell Factories·Lisa Ann BurdetteDanielle Tullman-Ercek
Nov 22, 2019·MRS Communications·Anton Kan, Neel S Joshi
Jan 3, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Gustavo Ruiz-Pulido, Dora I Medina
Feb 6, 2021·Mucosal Immunology·Bernadette Jones-FreemanMalcolm R Starkey
Mar 20, 2021·Nature Chemical Biology·Anna M Duraj-ThatteNeel S Joshi
Jan 11, 2021·Biotechnology Progress·Peter Tran, Arthur Prindle
Jun 19, 2021·Journal of Medicinal Chemistry·Nayara Braga EmidioMarkus Muttenthaler
Jul 18, 2021·Advanced Drug Delivery Reviews·Adriana Mejía-PittaCesar de la Fuente-Nunez
Dec 29, 2019·The Journal of Physical Chemistry. B·Sai Vamshi R JonnalagaddaManos Gkikas
May 24, 2018·ACS Synthetic Biology·Pichet PraveschotinuntNeel S Joshi
Aug 28, 2021·Biomedicines·Dan Cristian GheorgheAlexandru Mihai Grumezescu
Jun 11, 2018·ACS Biomaterials Science & Engineering·Eneko AxpeNeel S Joshi

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Methods Mentioned

BETA
ELISA
scraping

Software Mentioned

ImageJ

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