Modulating cell adhesion and spreading by control of FnIII7-10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates

Journal of Biomedical Materials Research. Part a
Hua WangShaoyi Jiang

Abstract

In this work, we demonstrate that surface charge can be used to modulate cell adhesion/spreading through the control of the orientation of adsorbed FnIII(7-10), which is a cell-adhesive protein containing RGD residues. Carboxylic acid (COOH) and amine (NH(2))-terminated self-assembled monolayers (SAMs) of alkanethiolates were used as model negatively and positively charged surfaces, respectively. The adsorbed amount of FnIII(7-10) is controlled to be equivalent on both SAMs as confirmed by the adsorption isotherms determined using I(125)-radiolabeled FnIII(7-10.) The binding of a monoclonal antibody specific for the cell-binding domain of FnIII(7-10) was measured by surface plasmon resonance (SPR) to evaluate FnIII(7-10) orientations on different SAMs. Results indicate that adsorbed FnIII(7-10) on NH(2)-SAM has an orientation with more cell-binding domains accessible than on COOH-SAM, confirming our predictions from Monte Carlo simulations. Both phase contrast images and Vybrant MTT cell proliferation assays show that the adhesion/spreading of bovine aortic endothelial cells (BAECs) on the NH(2)-SAM is significantly better than that on the COOH-SAM coated with an equivalent amount of FnIII(7-10). These results indicate that sur...Continue Reading

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Citations

Oct 20, 2011·Chemical Reviews·Steven R Meyers, Mark W Grinstaff
Nov 17, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Joo-Woon LeeChristine E Schmidt
Dec 15, 2010·Journal of the Royal Society, Interface·Manuel L B PalacioBharat Bhushan
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Jun 19, 2013·Journal of Biomedical Materials Research. Part a·Shawn RegisSankha Bhowmick
Jul 26, 2008·Journal of Biomedical Materials Research. Part a·K A BushG D Pins
Nov 16, 2007·Journal of Biomedical Materials Research. Part a·Cyrill BussyMarie-Danielle Nagel
Aug 13, 2013·Acta Biomaterialia·F Raquel MaiaCristina C Barrias
Nov 21, 2014·Physical Chemistry Chemical Physics : PCCP·Daohui ZhaoJian Zhou
Jan 11, 2008·Journal of Biomedical Materials Research. Part a·Matthew T Bernards, Shaoyi Jiang
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Apr 23, 2010·Colloids and Surfaces. B, Biointerfaces·Patricia RicoManuel Salmerón-Sánchez

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