Modulating Self-Assembly of DNA Crystals with Rationally Designed Agents

Angewandte Chemie
Jiemin ZhaoChengde Mao

Abstract

This manuscript reports a strategy for controlling the crystallization kinetics and improving the quality of engineered self-assembled 3D DNA crystals. Growing large, high-quality biomacromolecule crystals is critically important for determining the 3D structures of biomacromolecules. It often presents a great challenge to structural biologists. Herein, we introduce a rationally designed agent to modulate the crystallization process. Under such conditions, fewer, but larger, crystals that yield diffraction patterns of modestly higher resolution are produced compared with the crystals from conditions without the modulating agent. We attribute the improvement to a smaller number of nuclei and slow growth rate of crystallization. This strategy is expected to be generally applicable for crystallization of other biomacromolecules.

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Citations

Oct 22, 2020·Nanomaterials·Xuehui YanYe Tian
Jul 25, 2021·International Journal of Molecular Sciences·Shuang WangYe Tian
Jan 11, 2020·ACS Applied Materials & Interfaces·Chao XingHuanghao Yang
Sep 14, 2021·Journal of the American Chemical Society·Zhe LiChengde Mao

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