Modulation and redistribution of proteinase inhibitor 8 (Serpinb8) during kidney regeneration

American Journal of Nephrology
Anneliese GillardP I Bird

Abstract

The intracellular serpin, proteinase inhibitor 8 (PI8/Serpinb8), can inhibit furin, a prohormone convertase involved in inflammation, prohormone processing and extracellular matrix remodeling. Unilateral ureteral obstruction (UUO) is a well-characterized model of kidney disease associated with interstitial fibrosis, where recovery involves cellular proliferation and extracellular matrix remodeling. Given the presence of mouse PI8 (mPI8) in kidney, the UUO technique was used to investigate its potential role in kidney disease and regeneration. RT-PCR and in situ hybridization was used to assess PI8 mRNA in kidney sections. Immunohistochemistry was used to examine mPI8 distribution in normal kidney, and following reversal of UUO. mPI8 mRNA was detected in whole kidney by RT-PCR, and by in situ hybridization in convoluted tubules of the renal cortex and medulla. In normal and control contralateral unobstructed kidneys, mPI8 was within the ascending limb and convoluted section of the distal tubules. PI8 distribution did not change in UUO kidney, but was significantly altered in reversed UUO kidney, appearing in regions containing nephrons undergoing remodeling. These included regenerating proximal and distal tubules and glomeruli. ...Continue Reading

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Citations

Dec 15, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Kirstin M HeutinckAjda T Rowshani
Sep 11, 2008·Expert Opinion on Therapeutic Targets·Michel ChrétienMajambu Mbikay
Feb 18, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sami G MohammedJoost G J Hoenderop

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