PMID: 9185167Jul 1, 1997Paper

Modulation by both diphenyliodonium and diphenyleneiodonium of [3H]MK-801 binding to rat brain synaptic membranes

Neurochemistry International
M ShutoY Yoneda

Abstract

Binding of [3H](+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imi ne (MK-801) was significantly inhibited by the addition of several different compounds related to generation of nitric oxide (NO) at 100 microM in rat brain synaptic membranes. These included sodium nitroprusside, diphenyliodonium (DI), diphenyleneiodonium (DPI) and methylene blue. However, neither S-nitroso-N-acetylpenicillamine nor S-nitroso-L-glutathione inhibited binding at 100 microM. Both DI and DPI inhibited binding in a concentration-dependent manner at a concentration range of over 1 microM, while further addition of spermidine (SPD) significantly attenuated the potency of DPI to inhibit binding without affecting that of DI. In contrast, SPD induced significant potentiation of the ability of unlabelled MK-801 to displace [3H]MK-801 binding in a fashion sensitive to antagonism by the novel polyamine antagonist bis-(3-aminopropyl)nonanediamine. This novel polyamine antagonist also prevented the reversing effect of SPD on inhibition by DPI of [3H]MK-801 binding. Moreover, DPI competitively exacerbated the ability of SPD to potentiate [3H]MK-801 binding in the presence of both L-glutamic acid and glycine at maximally effective concentrations. On ...Continue Reading

References

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