Modulation by bradykinin and nitric oxide of angiotensin II-induced apoptosis in a vascular smooth muscle cell phenotype

International Immunopharmacology
S ColieN Blaes

Abstract

There is evidence for a clinical benefit of ACE inhibitors or AT1 antagonists in cardiovascular diseases with deleterious smooth muscle cells (SMC) apoptosis. We have previously shown that angiotensin II (Ang II) induces a phenotype-dependent SMC apoptosis. We asked whether bradykinin (BK) and nitric oxide (NO) could modulate Ang II-induced SMC apoptosis. BK alone did not induce significant apoptosis in either spindle (Sp-SMC) or epithelioid (Ep-SMC) SMC phenotypes cultured in serum reduction, but phenotype-dependently, reduced cell proliferation. Pretreatment with BK partly impaired Ang II-induced reduction of Ep-SMC culture viability and partly prevented apoptotic features. Pretreatment with sodium nitroprusside completely prevented all Ang II-induced deleterious effects in Ep-SMC, i. e. reduction of culture viability, Annexin V binding, nuclear condensation and cell fragmentation. These findings indicate that the BK-NO system may phenotype-dependently modulate SMC survival and in particular may oppose, mostly by NO, Ang II-induction of apoptosis in the Ep-SMC phenotype.

References

Sep 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N BlaesC Covacho
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L N WalkerM A Reidy
Apr 5, 1996·Biochemical and Biophysical Research Communications·E NishioY Watanabe
Nov 5, 1999·International Journal of Cardiology·S J PieperW K Shen
Nov 11, 1999·Cell Death and Differentiation·S Dimmeler, A M Zeiher
Feb 12, 2002·The American Journal of Medicine·Amir Halkin, Gad Keren
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Bradley S DixonMichael J Dennis
Sep 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Yong-Jian Geng, Peter Libby
Aug 9, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Hiroyuki HaoMarie-Luce Bochaton-Piallat
Jan 21, 2004·Cardiovascular Research·Shiow L PanChe M Teng
Dec 21, 2004·Biochemical and Biophysical Research Communications·Johan DucheneJoost P Schanstra
Feb 12, 2005·Antioxidants & Redox Signaling·Bernhard Brüne
Aug 30, 2005·American Journal of Hypertension·Bernard I Levy
Oct 5, 2006·Current Pharmaceutical Design·Jin Bo Su
Dec 13, 2006·American Journal of Nephrology·Murray Clarke, Martin Bennett

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