Modulation by LL-37 of the responses of salivary glands to purinergic agonists

Molecular Pharmacology
Stéphanie PochetJean-Paul Dehaye

Abstract

The interaction of mice submandibular gland cells with LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES), a cationic peptide with immunomodulatory properties, was investigated. LL-37 at a concentration that did not affect the integrity of the cells increased the uptake of calcium and activated a calcium-insensitive phospholipase A(2) (PLA(2)). The small release of ATP induced by LL-37 could not account for this stimulation because apyrase did not significantly block the response to LL-37. The divalent cation magnesium inhibited the response to LL-37, but this inhibition was probably nonspecific because it also inhibited the in vitro bacteriostatic effect of the peptide. The increase of calcium uptake by LL-37 was not affected by 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN-62), a rather specific inhibitor of P2X(7) receptors in mice. LL-37 also increased [Ca(2+)](i) in cells from mice invalidated for these receptors. LL-37 had no effect on the response to carbachol. It inhibited the increase of [Ca(2+)](i) and the activation of phospholipase D by ATP. It potentiated the activation of the PLA(2) by the nucleotide. Finally, LL-37 increased the fluidity of the plasma membrane of submandibular gland cel...Continue Reading

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Citations

Jan 6, 2010·Archivum Immunologiae Et Therapiae Experimentalis·Robert BuckiWojciech Sokołowski
Mar 28, 2008·Purinergic Signalling·I Novak
Nov 26, 2009·Natural Product Reports·Matthew F Burton, Patrick G Steel
Jan 1, 2014·Journal of Clinical Periodontology·Anupong MakeudomSuttichai Krisanaprakornkit
Nov 12, 2015·Biochimica Et Biophysica Acta·Daniela XhindoliAlessandro Tossi
Mar 17, 2009·Biochimica Et Biophysica Acta·Christian CodePaavo K J Kinnunen
Jun 12, 2013·Biochemical and Biophysical Research Communications·Ajay K Mahalka, Paavo K J Kinnunen
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Oct 25, 2016·Peptides·Eddy-Tim VerjansLiliane Schoofs
Jul 4, 2006·Cellular Signalling·Mikel Garcia-MarcosJean-Paul Dehaye

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