Modulation by substrates of the interaction between the HasR outer membrane receptor and its specific TonB-like protein, HasB

Journal of Molecular Biology
Julien LefèvreNadia Izadi-Pruneyre

Abstract

TonB is a cytoplasmic membrane protein required for active transport of various essential substrates such as heme and iron siderophores through the outer membrane receptors of Gram-negative bacteria. This protein spans the periplasm, contacts outer membrane transporters by its C-terminal domain, and transduces energy from the protonmotive force to the transporters. The TonB box, a relatively conserved sequence localized on the periplasmic side of the transporters, has been shown to directly contact TonB. While Serratia marcescens TonB functions with various transporters, HasB, a TonB-like protein, is dedicated to the HasR transporter. HasR acquires heme either freely or via an extracellular heme carrier, the hemophore HasA, that binds to HasR and delivers heme to the transporter. Here, we study the interaction of HasR with a HasB C-terminal domain and compare it with that obtained with a TonB C-terminal fragment. Analysis of the thermodynamic parameters reveals that the interaction mode of HasR with HasB differs from that with TonB, the difference explaining the functional specificity of HasB for HasR. We also demonstrate that the presence of the substrate on the extracellular face of the transporter modifies, via enthalpy-entr...Continue Reading

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Citations

Feb 28, 2013·BMC Biology·Grace E Kenney, Amy C Rosenzweig
Mar 26, 2013·PloS One·Gisele Cardoso de AmorimNadia Izadi-Pruneyre
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Oct 22, 2013·Biochimica Et Biophysica Acta·Allan MillsFranck Duong
Apr 21, 2017·Annual Review of Biochemistry·Weiliang Huang, Angela Wilks
Jan 12, 2019·PloS One·Lokender KumarSusanta K Sarkar
Sep 28, 2017·Frontiers in Cellular and Infection Microbiology·Nick D PokorzynskiRey A Carabeo
Jan 23, 2009·Journal of the American Chemical Society·Célia Caillet-SaguyAnne Lecroisey
Nov 4, 2008·Archives of Biochemistry and Biophysics·Yong Tong, Maolin Guo

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