Modulation in cell cycle and cyclin B1 expression in irradiated HeLa cells and normal human skin fibroblasts treated with staurosporine and caffeine

Experimental Cell Research
P K NarayananH A Crissman

Abstract

The comparative effects of staurosporine or caffeine on G2-phase arrest and cyclin B1 expression in human skin fibroblasts (HSF) and transformed HeLa cells following gamma-irradiation were examined by flow cytometry. Contrary to some earlier reports with HeLa cells, the arrest in G2 after irradiation was accompanied by an increase in cyclin B1 levels in both asynchronous and synchronized HeLa cells irradiated in early S phase. Caffeine and staurosporine were equally effective in attenuating both the radiation-induced increase in cyclin B1 expression and the prolongation of G2 in synchronous and asynchronous HeLa cell populations. Staurosporine treatment was less effective in down-regulating cyclin B1 expression in asynchronous HeLa cells at earlier time points following irradiation when compared to caffeine-treated cells. In synchronized HeLa cells, down-regulation of an irradiation-induced increase in cyclin B1 expression was similar to either staurosporine or caffeine treatments, with caffeine being more effective at later time points. An increase in cyclin B1 expression was also observed in irradiated HSF cells (synchronous and asynchronous), which decreased when the cells were treated with staurosporine or caffeine. However...Continue Reading

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Citations

Oct 20, 2011·Radiation Oncology·Susanne AuerAnna A Friedl
Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·G DeplanqueC Klein-Soyer
Mar 8, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gopalan NatarajanShrikant Anant
Nov 9, 2007·Acta Biochimica Et Biophysica Sinica·Xinchen SunYingfeng Zhou

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