Modulation of a membrane lipid lamellar-nonlamellar phase transition by cationic lipids: a measure for transfection efficiency

Biochimica Et Biophysica Acta
Boris TenchovRobert C MacDonald

Abstract

Synthetic cationic lipids can be used as DNA carriers and are regarded to be the most promising non-viral gene carriers. For this investigation, six novel phosphatidylcholine (PC) cationic derivatives with various hydrophobic moieties were synthesized and their transfection efficiencies for human umbilical artery endothelial cells (HUAEC) were determined. Three compounds with relatively short, myristoleoyl or myristelaidoyl 14:1 chains exhibited very high activity, exceeding by approximately 10 times that of the reference cationic derivative dioleoyl ethylPC (EDOPC). Noteworthy, cationic lipids with 14:1 hydrocarbon chains have not been tested as DNA carriers in transfection assays previously. The other three lipids, which contained oleoyl 18:1 and longer chains, exhibited moderate to weak transfection activity. Transfection efficiency was found to correlate strongly with the effect of the cationic lipids on the lamellar-to-inverted hexagonal, Lalpha-->HII, phase conversion in dipalmitoleoyl phosphatidylethanolamine dispersions (DPoPE). X-ray diffraction on binary DPoPE/cationic lipid mixtures showed that the superior transfection agents eliminated the direct Lalpha-->HII phase transition and promoted formation of an inverted c...Continue Reading

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Citations

May 16, 2012·European Biophysics Journal : EBJ·Boris Tenchov, Rumiana Koynova
Apr 4, 2009·Molecular Pharmaceutics·Rumiana KoynovaRobert C Macdonald
Dec 8, 2009·Biochemistry. Biokhimii︠a︡·Y S Tarahovsky
Jul 16, 2014·Colloids and Surfaces. B, Biointerfaces·João P Neves SilvaM Elisabete C D Real Oliveira
Dec 3, 2015·Bioconjugate Chemistry·Maneesh GujratiZheng-Rong Lu
Oct 15, 2011·Biophysical Chemistry·Petra PullmannováDaniela Uhríková
Nov 1, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Christopher JanichAndreas Langner
Feb 20, 2016·Organic & Biomolecular Chemistry·P Parvizi-BahktarT M Fyles
Oct 29, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Philippe Pierrat, Luc Lebeau
Mar 1, 2012·Chemistry : a European Journal·Philippe PierratLuc Lebeau
Nov 20, 2009·International Journal of Pharmaceutics·Cai-Xia HeJian-Qing Gao
Jan 9, 2013·Chemistry : a European Journal·Philippe PierratLuc Lebeau
May 12, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian WölkGerald Brezesinski
Jun 22, 2016·Soft Matter·Christopher JanichChristian Wölk
Nov 5, 2019·Biochimica Et Biophysica Acta. Biomembranes·Marzena MachPaweł Wydro
Oct 7, 2017·Chemistry and Physics of Lipids·Boris Tenchov, Rumiana Koynova
May 13, 2017·Current Opinion in Colloid & Interface Science·Minjee KangCecilia Leal
Jul 20, 2021·Chemistry and Physics of Lipids·Nina Královič-KanjakováDaniela Uhríková
Jul 17, 2012·Molecular Pharmaceutics·Guoliang ZhenPathiraja Gunatillake
Mar 9, 2015·ACS Biomaterials Science & Engineering·Rumiana KoynovaRobert C MacDonald
Sep 6, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Christopher JanichChristian Wölk
Sep 21, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Gilda LiskayováDaniela Uhríková

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