PMID: 7541368Jul 1, 1995Paper

Modulation of adhesion molecule CD54 expression on CD34+ hematopoietic progenitors

Experimental Hematology
S ArkinJ M Lipton

Abstract

Multiple cellular adhesion molecules are constitutively expressed on hematopoietic progenitors. CD54 (ICAM-1), a member of the immunoglobulin supergene family, has recently been shown to be present on a subset of hematopoietic progenitors. To demonstrate that this adhesion molecule is expressed on the surface of the hematopoietic progenitor following induction rather than in its basal state, we studied the expression of cell surface CD54 on flow-cytometric (FACS) gated progenitors (CD34+; lymph plus blast light scatter). A mean +/- standard deviation (SD) of 2.75 +/- 2.10% was seen for FACS-gated progenitors from freshly isolated bone marrow mononuclear cells (BMMNC) stained with anti-CD54 MAb. Following incubation at 37 degrees C for 20 to 22 hours in media supplemented with heat-inactivated bovine fetal serum (FBS), the percentage of FACS-gated progenitors staining with anti-CD54 MAb increased to 46.28 +/- 18.38. This increase was temperature and time dependent. The percentage of FACS-gated progenitors stained with anti-CD54 MAb did not increase following 20 to 22 hours of incubation at 4 degrees C. Furthermore, a subpopulation of FACS-gated progenitors staining with anti-CD54 MAb was detectable by 5 hours of incubation at 37...Continue Reading

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