PMID: 8613485Nov 1, 1995Paper

Modulation of adhesion molecules by cytokines in vivo using human/severe combined immunodeficient (SCID) mouse chimeras

Journal of Clinical Immunology
J M PilewskiS M Albelda

Abstract

Endothelial cell-leukocyte interactions involve multiple cell adhesion molecules acting in a programmed and sequential manner to create a leukocyte-endothelial cell adhesion cascade. To understand this process fully, in vivo models are needed. To accomplish this, we have transplanted pieces of normal human tissues onto immunodeficient mice to create chimeric animals. In one model, human skin is grafted and closely resembles normal skin histologically. The grafts retain their human vasculature and show low baseline expression of E-selectin, vascular cell adhesion molecule-1, and intercellular cell adhesion molecule-1. After intradermal injection of human cytokines, these cell adhesion molecules are markedly upregulated and an active inflammatory reaction ensues, with migration of murine leukocytes. Intravenous injection of an anti-human E-selectin antibody completely inhibits leukocyte accumulation induced by tumor necrosis factor-alpha but only partially inhibits leukotriene B4-induced inflammation. In a second model, human bronchus was successfully transplanted heterotopically into severe combined immunodeficient mice. Injection of tumor necrosis factor induced upregulation of E-selectin, intercellular cell adhesion molecule-1...Continue Reading

References

May 1, 1991·The Journal of Clinical Investigation·M A Perry, D N Granger
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·U H von AndrianE C Butcher
Oct 1, 1991·The Journal of Clinical Investigation·M S MulliganP A Ward
Apr 1, 1990·Immunological Reviews·T M Carlos, J M Harlan
Oct 1, 1990·Transplantation·J S Pober, R S Cotran
Oct 1, 1989·The Journal of Investigative Dermatology·J N BarkerD M MacDonald
Nov 1, 1989·The Journal of Investigative Dermatology·R E LewisG F Murphy
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Feb 1, 1984·The Journal of Investigative Dermatology·R CampM Greaves
Sep 1, 1994·American Journal of Respiratory and Critical Care Medicine·J M PilewskiS M Albelda
Feb 1, 1993·The Journal of Clinical Investigation·M P Bevilacqua, R M Nelson
Feb 1, 1993·The Journal of Clinical Investigation·M S MulliganP A Ward
Aug 1, 1993·The Journal of Experimental Medicine·W A MullerD M Phillips

❮ Previous
Next ❯

Citations

Apr 9, 2001·Infection and Immunity·W F StensonS L Stanley
Jun 1, 2006·Expert Opinion on Drug Discovery·Frederik H IgneyThomas M Zollner
Feb 28, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Benjamin R ShepherdJames B Hoying

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.