PMID: 11341970May 9, 2001Paper

Modulation of alpha2beta1 integrin changes during mammary gland development by beta-oestradiol

Biochimica Et Biophysica Acta
T IypeP R Sudhakaran

Abstract

In order to study the role of cell-matrix interactions in mammary gland function, temporal changes in alpha2beta1 integrin, the major receptor for collagen and the influence of beta-oestradiol on its level and distribution in rat mammary gland at different stages of development were studied. The level of alpha2beta1 integrin determined by ELISA, was found to be high during different days of pregnancy, while in the lactating stage, it was significantly reduced. By immunocytochemical analysis, alpha2beta1 integrin was found to be localized towards the luminal side of acinar cells, both in the virgin and midpregnant stage, while it was not detected in the lactating stage. The possible role of hormones in modulating the level of integrin was examined in both in vitro and in vivo experiments using beta-oestradiol. Supplementing beta-oestradiol to isolated mammary epithelial cells from both virgin and lactating glands caused a concentration dependent increase in the incorporation of [35S]methionine into alpha2beta1 integrin associated with the cells. Administration of beta-oestradiol to virgin and lactating glands caused about 1.4-4-fold increase in the level of alpha2 integrin, indicating that upregulation of integrin during pregnan...Continue Reading

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Citations

Jun 11, 2003·Journal of Clinical Periodontology·M Soory, A Tilakaratne
Aug 24, 2002·Biology of Reproduction·Kathleen H BurnsMartin M Matzuk
Aug 17, 2006·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Barbara NebeVolker Briese

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