Modulation of AMPA receptor-mediated ion current by pituitary adenylate cyclase-activating polypeptide (PACAP) in CA1 pyramidal neurons from rat hippocampus

Hippocampus
L CostaL Ciranna

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP), a neurotrophic and neuromodulatory peptide, was recently shown to enhance NMDA receptor-mediated currents in the hippocampus (Macdonald, et al. 2005. J Neurosci 25:11374-11384). To check if PACAP might also modulate AMPA receptor function, we tested its effects on AMPA receptor-mediated synaptic currents on CA1 pyramidal neurons, using the patch clamp technique on hippocampal slices. In the presence of the NMDA antagonist D-AP5, PACAP (10 nM) reduced the amplitude of excitatory postsynaptic currents (EPSCs) evoked in CA1 pyramidal neurons by stimulation of Schaffer collaterals. Following a paired-pulse stimulation protocol, the paired-pulse ratio was unaffected in most neurons, suggesting that the AMPA-mediated EPSC was modulated by PACAP mainly at a postsynaptic level. PACAP also modulated the currents induced on CA1 pyramidal neurons by applications of either glutamate or AMPA. The effects of PACAP were dose-dependent: at a 0.5 nM dose, PACAP increased AMPA-mediated current; such effect was blocked by PACAP 6-38, a selective antagonist of PAC1 receptors. The enhancement of AMPA-mediated current by PACAP 0.5 nM was abolished when cAMPS-Rp, a PKA inhibitor, was added t...Continue Reading

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Citations

Apr 24, 2010·Journal of Molecular Neuroscience : MN·Kai YangJohn F Macdonald
Oct 1, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jon M ReschSujean Choi
May 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alyssa M A Toda, Richard L Huganir
Mar 10, 2011·Journal of Chemical Neuroanatomy·Adalberto MerighiLaura Lossi
Dec 10, 2014·Neurobiology of Learning and Memory·S D SchmidtI Izquierdo
Jun 5, 2019·Annals of the New York Academy of Sciences·Gregory C JohnsonSayamwong E Hammack
May 20, 2020·Frontiers in Cellular Neuroscience·Gregory C JohnsonSayamwong E Hammack
Feb 14, 2012·Journal of Cell Science·Fabrizio GardoniMonica Di Luca
Jul 10, 2021·Frontiers in Cellular Neuroscience·Marieke R Gilmartin, Nicole C Ferrara
Nov 30, 2021·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Samyak Pandey, Jayesh Mudgal

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