Modulation of AMPA receptors in spinal motor neurons by the neuroprotective agent riluzole

Journal of Neuroscience Research
Federica AlboC Zona

Abstract

We investigated the interaction of riluzole, a therapeutic agent used in amyotrophic lateral sclerosis (ALS), with alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor channels in mouse spinal motor neurons in culture using whole-cell patch-clamp recording techniques. Kainate elicited concentration-dependent (EC(50) = 35 microM) inward currents in all the patched cells. These responses were mediated primarily through the activation of AMPA receptors with a negligible contribution from kainate receptors, because bath application of 100 microM GYKI53655, a potent noncompetitive AMPA receptor antagonist, completely blocked the kainate-induced currents. Riluzole (0.5-100 microM) reduced in a dose-dependent manner the kainate-induced currents with an IC(50) of 1.54 microM in all tested neurons (n = 25) and this effect was found to be reversible. The response to kainate decreased in the presence of 1 microM riluzole in all spinal motor neurons tested, without changing its EC(50), indicating a noncompetitive mechanism of inhibition. The amplitude of the responses induced by kainate under control condition and during riluzole was a linear function of the membrane potential. The reversal potential of the current was...Continue Reading

References

Dec 1, 1991·Pflügers Archiv : European journal of physiology·E Benoit, D Escande
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 1, 1995·Neuropharmacology·B Bettler, C Mulle
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
Mar 3, 1994·The New England Journal of Medicine·G BensimonV Meininger
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·B LambolezS Hestrin
Jun 1, 1997·Trends in Pharmacological Sciences·E LouvelA Doble
Apr 29, 1998·Journal of Clinical Pharmacology·A Le LibouxG Montay
Mar 26, 1999·Movement Disorders : Official Journal of the Movement Disorder Society·H D RosasM E Cudkowicz
Oct 13, 2000·The European Journal of Neuroscience·A Urbani, O Belluzzi
Nov 25, 2000·Journal of the Neurological Sciences·L Van Den BoschW Robberecht
Aug 7, 2002·European Journal of Pharmacology·Ying-Jun CaoKhaled M Houamed
Apr 1, 2003·Annals of Neurology·Jasna KrizJean-Pierre Julien
Oct 3, 2003·Journal of Neurophysiology·Jason J KuoAlfons B A Kroese

❮ Previous
Next ❯

Citations

Sep 14, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Christopher Pittenger, Ronald S Duman
Apr 12, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Maria DimitriadiAnne C Hart
Oct 23, 2013·Neurología : publicación oficial de la Sociedad Española de Neurología·U Gomez-PinedoA G García
Aug 23, 2011·Pharmacological Reports : PR·Kambiz HassanzadehSadollah Mohammadi
Dec 21, 2010·Experimental Neurology·Marin Manuel, C J Heckman
May 12, 2009·Neuroscience Letters·Alison StevensonChristopher C J Miller
Nov 27, 2007·European Journal of Pharmacology·Elena FumagalliTiziana Mennini
Jun 15, 2007·The European Journal of Neuroscience·Sabrina ReimersSteffen Rossner
Feb 2, 2006·Journal of Physiology, Paris·Jacques DurandBoris Lamotte d'Incamps
Sep 23, 2009·Neurobiology of Disease·Hanna WootzKlas Kullander
Apr 21, 2009·Biochemical Pharmacology·Gerald W Valentine, Gerard Sanacora
Jun 30, 2006·Biochimica Et Biophysica Acta·L Van Den BoschW Robberecht
Dec 31, 2016·Journal of Alzheimer's Disease : JAD·Mohammad Asif Emran Khan EmonMartin Hofmann-Apitius
Oct 15, 2005·Revista brasileira de psiquiatria : orgão oficial da Associação Brasileira de Psiquiatria, Asociación Psiquiátrica de la América Latina·Sanjay J MathewLisa Smith
Dec 10, 2017·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration·Marion C HoggJochen H M Prehn
Aug 20, 2009·Anesthesia and Analgesia·Bohlool Habibi-AslMohammad Charkhpour
Jul 12, 2008·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Steven J Del SignoreRobert J Ferrante
Mar 9, 2007·Journal of Neurophysiology·Cédric YvonJürg Streit
Aug 14, 2008·CNS Drugs·Christopher PittengerGerard Sanacora
Feb 27, 2017·Journal of Molecular Neuroscience : MN·Giovanna MorelloSebastiano Cavallaro
Aug 15, 2021·The European Journal of Neuroscience·Amanda L WrightAdam K Walker

❮ Previous
Next ❯

Related Concepts

Related Feeds

ALS: Therapies

Amyotrophic Lateral Sclerosis (ALS) is associated with the death of neurons that control voluntary muscles. This feed followes the latest research into therapies for this progressive neurodegenerative disease.

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.