Modulation of amplitude and direction of in vivo immune responses by co-administration of cytokine gene expression cassettes with DNA immunogens

European Journal of Immunology
J J KimD B Weiner

Abstract

Immunization with nucleic acids has been shown to induce both antigen-specific cellular and humoral immune responses in vivo. We hypothesize that immunization with DNA could be enhanced by directing specific immune responses induced by the vaccine based on the differential correlates of protection known for a particular pathogen. Recently we and others reported that specific immune responses generated by DNA vaccine could be modulated by co-delivery of gene expression cassettes encoding for IL-12, granulocyte-macrophage colony-stimulating factor and the co-stimulatory molecule CD86. To further engineer the immune response in vivo, we investigated the induction and regulation of immune responses following the co-delivery of pro-inflammatory cytokine (IL-1 alpha, TNF-alpha, and TNF-beta), Th1 cytokine (IL-2, IL-12, IL-15, and IL-18), and Th2 cytokine (IL-4, IL-5 and IL-10) genes. We observed enhancement of antigen-specific humoral response with the co-delivery of Th2 cytokine genes IL-4, IL-5, and IL-10 as well as those of IL-2 and IL-18. A dramatic increase in antigen-specific T helper cell proliferation was seen with IL-2 and TNF-alpha gene co-injections. In addition, we observed a significant enhancement of the cytotoxic respo...Continue Reading

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Citations

Nov 13, 2004·The Journal of Gene Medicine·Gérald J Prud'homme
Oct 21, 2006·Springer Seminars in Immunopathology·Franco LoriJulianna Lisziewicz
Dec 11, 2008·Immunologic Research·Shaheed A Abdulhaqq, David B Weiner
Feb 13, 2001·Molecular Immunology·G S BuchanM A Baird
Mar 21, 2001·Vaccine·J Y Scheerlinck
Jan 18, 2003·Vaccine·Mark T EsserMichael W Washabaugh
Jan 26, 2000·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·M GirardC Chanel
May 26, 1999·Journal of Dermatological Science·A Morita, A Takashima
Nov 30, 2005·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Ephraim L Tsalik
Dec 14, 1999·Microbes and Infection·H L Davis, M J McCluskie
Apr 5, 2001·Trends in Immunology·G J Prud'hommeA N Theofilopoulos
May 10, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Vivek ChitnisSavita Pahwa
Mar 22, 2002·Nature Reviews. Immunology·J A BerzofskyI M Belyakov
May 7, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dorian McIlroyBruno Pitard
Dec 19, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michael A ChattergoonDavid B Weiner
Dec 19, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B Spellberg, J E Edwards
Jun 14, 2001·DNA and Cell Biology·A CanoK Manoutcharian
Apr 13, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J J KimD B Weiner
Jun 7, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J J KimD B Weiner
Feb 27, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J J KimD B Weiner

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