Modulation of apoptosis in intestinal lymphocytes by a probiotic bacteria in Crohn's disease

Journal of Leukocyte Biology
M CarolJuan-R Malagelada

Abstract

Apoptosis of active T lymphocytes constitutes a major control mechanism of immune homeostasis and tolerance. In Crohn's disease, abnormal activation of mucosal T lymphocytes against enteric bacteria is the key event triggering intestinal inflammation. Resistance of lymphocytes to apoptosis has been proposed as the pathogenetic defect. We examined the influence of bacteria-mucosa interactions on apoptosis of mucosal T lymphocytes. Ileal specimens were obtained at surgery from 12 patients with Crohn's disease. Mucosal explants from each specimen were cultured with nonpathogenic Escherichia coli ATCC 35345, Lactobacillus casei DN-114 001, or no bacteria. Cytokine release was measured in supernatant, and mononuclear cells were isolated for phenotypic characterization and Bcl-2 family protein expression. Coculture of inflamed tissue with L. casei significantly reduced the release of interleukin (IL)-6 and tumor necrosis factor alpha (P < 0.05). In addition, coculture with L. casei significantly reduced the number of T cells displaying the IL-2 receptor in the lamina propria. Expression of the antiapoptotic protein Bcl-2 in lamina propria lymphocytes was also reduced after coculture with L. casei, and the percentage of deoxyuridine t...Continue Reading

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Citations

Oct 8, 2008·Inflammatory Bowel Diseases·Marta LlopisJuan-R Malagelada
Apr 2, 2008·Current Gastroenterology Reports·R Balfour Sartor, Marcus Muehlbauer
Dec 11, 2013·Cellular Immunology·Sean BandzarManu O Platt
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Jan 4, 2021·Advanced Drug Delivery Reviews·Abdallah LadayciaElise Lepeltier

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