PMID: 9443068Jan 27, 1998Paper

Modulation of apoptosis induced by tricyclic antidepressants in human peripheral lymphocytes

Journal of Biochemical and Molecular Toxicology
Z XiaL Nässberger

Abstract

We have recently reported that the tricyclic antidepressants (TCAs) imipramine, clomipramine, and citalopram induce apoptosis in human peripheral lymphocytes. This system is well suited for studies on the pathophysiology/physiology of apoptosis regulation. Apoptosis was determined using both DNA gel electrophoresis and flow cytometric analysis. TCA-induced apoptosis in lymphocytes was monitored in the presence of the protein synthesis inhibitor cycloheximide (CHX), the RNA synthesis inhibitor actinomycin D (Act D), the antioxidant reduced glutathione (GSH), the nuclease inhibitor aurintricarboxylic acid (ATA), the cytokine interleukin-2 (IL-2), and the immunostimulator linomide. CHX and Act D failed to prevent and actually enhanced TCA-induced apoptosis in lymphocytes, indicating that protein and RNA syntheses are not required for this process. Exogenous IL-2, GSH, and ATA protected the lymphocytes from apoptosis induced by TCAs in a dose-dependent manner, whereas linomide had no effect on TCA-induced apoptosis under our in vitro conditions. Our data demonstrate that TCA-induced apoptosis in human lymphocytes shares many common features with other stimuli-induced apoptotic processes.

Citations

Dec 18, 2013·European Journal of Medicinal Chemistry·Y M McNamaraM J Meegan
Aug 12, 2003·Trends in Immunology·John Gordon, Nicholas M Barnes
Dec 21, 2000·The European Journal of Neuroscience·A PostC Behl
Nov 7, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Si ChenLei Guo
May 27, 2010·International Journal of Cancer. Journal International Du Cancer·Suzanne M Cloonan, David Clive Williams
Jun 7, 2006·European Journal of Pharmacology·Hideki Arimochi, Kyoji Morita
Feb 2, 2013·Life Sciences·Luciana Romina Frick, Maximiliano Rapanelli
Sep 26, 2017·Cancer Treatment and Research Communications·Adriana ZingoneBríd M Ryan

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis