Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression

Applied and Environmental Microbiology
Marta LombrañaJuan F Martín

Abstract

Two different strains, Aspergillus awamori TGDTh-4 and A. awamori TGP-3 overexpressing a synthetic gene encoding the plant sweet protein thaumatin, showed an unfolded protein response. To facilitate protein secretion, the chaperone BiPA gene was expressed in A. awamori under control of the strong constitutive promoter of the gpdA gene. A good correlation was observed between the level of the bipA transcript in different strains and the amount of thaumatin secreted. Thaumatin secretion was increased 2- to 2.5-fold in transformants overexpressing the bipA gene compared with the parental strain. Secretion of the homologous proteins alpha-amylase and glucoamylase was not affected by the bipA gene overexpression. The requirement for BiPA for secretion of thaumatin was confirmed by attenuation of the endogenous bipA gene expression with an antisense RNA cassette. The decrease in bipA expression reduced the amount of secreted thaumatin up to 80% without affecting the secretion of the homologous alpha-amylase and glucoamylase proteins. The BiPA protein is, therefore, very important for secretion of some heterologous proteins, such as thaumatin in A. awamori.

References

Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M M YeltonW E Timberlake
Aug 1, 1995·Current Opinion in Cell Biology·C Hammond, A Helenius
Dec 1, 1994·Protein Expression and Purification·T A HsuM J Betenbaugh
Apr 26, 1996·The Journal of Biological Chemistry·A S RobinsonK D Wittrup
Jan 1, 1997·Applied Microbiology and Biotechnology·R J GoukaC A van den Hondel
Dec 31, 1997·The Journal of Biological Chemistry·J C Morris, K Mensa-Wilmot
Jan 1, 1997·Critical Reviews in Biotechnology·D B Archer, J F Peberdy
Apr 18, 1998·Biochimica Et Biophysica Acta·D TechelL Rensing
Aug 4, 1998·Applied and Environmental Microbiology·I A van GemerenC T Verrips
Aug 10, 2001·Fungal Genetics and Biology : FG & B·A ConesaC A van den Hondel
Feb 2, 2002·Applied and Environmental Microbiology·Ana ConesaPeter J Punt

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Citations

Feb 9, 2010·Applied Microbiology and Biotechnology·Alimjan IdirisKaoru Takegawa
Jun 10, 2010·Applied Microbiology and Biotechnology·André Fleissner, Petra Dersch
Jun 19, 2012·Applied Microbiology and Biotechnology·Marzena BlumhoffMichael Sauer
Sep 15, 2005·Molecular Genetics and Genomics : MGG·D A MacKenzieD B Archer
Jun 4, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tetsuya MasudaNaofumi Kitabatake
Dec 18, 2007·Applied and Environmental Microbiology·Elke KainzJoseph Strauss
Mar 12, 2016·Protein Expression and Purification·Aleksey FirsovSergey Dolgov
Dec 26, 2006·Journal of Bioscience and Bioengineering·Tetsuya Masuda, Naofumi Kitabatake
Sep 28, 2007·Biochemical and Biophysical Research Communications·Nobuyuki IdeNaofumi Kitabatake
Nov 12, 2014·Applied Microbiology and Biotechnology·Kai Heimel
Apr 24, 2020·Frontiers in Bioengineering and Biotechnology·Qin WangHan Xiao
Jul 23, 2020·Bioscience, Biotechnology, and Biochemistry·Shinobu TakagiShinichi Ohashi
Mar 23, 2019·World Journal of Microbiology & Biotechnology·Xianhua Sun, Xiaoyun Su
Apr 27, 2019·Frontiers in Microbiology·Jewel Ann JosephJan F M Van Impe

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