Modulation of autophagy and ubiquitin-proteasome pathways during ultra-endurance running

Journal of Applied Physiology
Cécile JamartLéonard Féasson

Abstract

In this study, the coordinated activation of ubiquitin-proteasome pathway (UPP), autophagy-lysosomal pathway (ALP), and mitochondrial remodeling including mitophagy was assessed by measuring protein markers during ultra-endurance running exercise in human skeletal muscle. Eleven male, experienced ultra-endurance athletes ran for 24 h on a treadmill. Muscle biopsy samples were taken from the vastus lateralis muscle 2 h before starting and immediately after finishing exercise. Athletes ran 149.8 ± 16.3 km with an effective running time of 18 h 42 min ( ± 41 min). The phosphorylation state of Akt (-74 ± 5%; P < 0.001), FOXO3a (-49 ± 9%; P < 0.001), mTOR Ser2448 (-32 ± 14%; P = 0.028), and 4E-BP1 (-34 ± 7%; P < 0.001) was decreased, whereas AMPK phosphorylation state increased by 247 ± 170% (P = 0.042). Proteasome β2 subunit activity increased by 95 ± 44% (P = 0.028), whereas the activities associated with the β1 and β5 subunits remained unchanged. MuRF1 protein level increased by 55 ± 26% (P = 0.034), whereas MAFbx protein and ubiquitin-conjugated protein levels did not change. LC3bII increased by 554 ± 256% (P = 0.005), and the form of ATG12 conjugated to ATG5 increased by 36 ± 17% (P = 0.042). The mitochondrial fission marker ph...Continue Reading

References

May 1, 1968·The American Journal of Physiology·P H Wright, W J Malaisse
Apr 1, 1994·International Journal of Sports Medicine·H Kuipers
Jun 17, 1998·The Journal of Biological Chemistry·J T Brozinick, M J Birnbaum
Mar 9, 2002·The Journal of Biological Chemistry·Thomas H ReynoldsJohn C Lawrence
Aug 8, 2002·Medicine and Science in Sports and Exercise·Gregoire P MilletRobin Candau
May 16, 2003·Current Biology : CB·Galina PolekhinaDavid Stapleton
Jan 14, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stewart H LeckerAlfred L Goldberg
Jun 24, 2004·Nutrition·Mark Tarnopolsky
May 12, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael B Reid
May 24, 2005·Current Sports Medicine Reports·Calvin Zaryski, David J Smith
Nov 4, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vernon G CoffeyJohn A Hawley
May 18, 2006·Annual Review of Cell and Developmental Biology·David C Chan
Sep 23, 2006·The Journal of Biological Chemistry·Daniel MeleyAlfred J Meijer
Nov 11, 2006·Medicine and Science in Sports and Exercise·William W WinderDavid M Thomson
Aug 23, 2007·The Journal of Biological Chemistry·Eric L GreerAnne Brunet
Dec 7, 2007·Cell Metabolism·Cristina MammucariMarco Sandri
Apr 2, 2008·Medicine and Science in Sports and Exercise·Matthew A PikoskyAndrew J Young
Apr 5, 2008·Mediators of Inflammation·Vitaliy PoylinPer-Olof Hasselgren
Jun 7, 2008·European Journal of Applied Physiology·Louise DeldicqueMarc Francaux
Jun 17, 2008·Physiology·Marco Sandri
Aug 19, 2008·Biochimica Et Biophysica Acta·Eeva-Liisa Eskelinen, Paul Saftig
Oct 2, 2008·American Journal of Physiology. Endocrinology and Metabolism·Boubacar BenzianeNigel K Stepto
Oct 30, 2008·Biochemical and Biophysical Research Communications·Yongsung KimJongkyeong Chung
Jan 2, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Matthew P HarberScott W Trappe
Feb 21, 2009·Cell Death and Differentiation·J Zhang, P A Ney
Nov 4, 2009·Scandinavian Journal of Medicine & Science in Sports·G Y MilletL Feasson
Dec 1, 2009·Cell Metabolism·Eva MasieroMarco Sandri
Jan 20, 2010·FEBS Letters·Chang Hwa JungDo-Hyung Kim

❮ Previous
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Citations

Jul 31, 2013·Trends in Molecular Medicine·Vladimir Ljubicic, Bernard J Jasmin
Nov 26, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Anna VainshteinPaolo Bonaldo
Jan 24, 2014·Antioxidants & Redox Signaling·Elisabetta FerraroGiuseppe Rosano
Nov 16, 2013·Cellular and Molecular Life Sciences : CMLS·Anthony M J SanchezHenri Bernardi
Feb 20, 2014·Sports Medicine·Bjorn T Tam, Parco M Siu
Feb 14, 2016·Free Radical Research·Alex J WadleySarah Aldred
Nov 13, 2015·Expert Opinion on Investigational Drugs·F PennaP Costelli
Feb 16, 2016·Free Radical Biology & Medicine·Donny M CameraJohn A Hawley
Nov 29, 2015·The Journal of Physiology·Andreas M FritzenChristian Frøsig
Mar 5, 2016·Age·Yubisay Mejías-PeñaJavier González-Gallego
Dec 10, 2015·Nature Medicine·Susmita Kaushik, Ana Maria Cuervo
Nov 14, 2015·Seminars in Cell & Developmental Biology·Marco Sandri
Apr 8, 2016·Skeletal Muscle·Ryan A V BellLynn A Megeney
May 15, 2013·The International Journal of Biochemistry & Cell Biology·Marco Sandri
Apr 22, 2014·Neuromuscular Disorders : NMD·Luciano MerliniUNKNOWN Consortium for Autophagy in Muscular Dystrophies
Apr 4, 2015·Autophagy·Louis R LapierreMalene Hansen
Feb 26, 2015·Autophagy·Karol DokladnyPope L Moseley
Dec 3, 2013·Biochimica Et Biophysica Acta·Aaron P RussellGlenn D Wadley
Dec 9, 2014·Biochemical and Biophysical Research Communications·Dong-Mei ZhengJunji Ezaki
Aug 12, 2014·Journal of Bioenergetics and Biomembranes·J Eva SelfridgeRussell H Swerdlow
May 21, 2016·Frontiers in Physiology·Bill TachtsisDonny M Camera
Jul 19, 2016·Bioscience, Biotechnology, and Biochemistry·Tomonori SatoTakashi Nagasawa
Sep 16, 2016·The Journal of Physiology·Anthony M J Sanchez
Oct 28, 2015·The Journal of Physiology·Anna-Maria JosephChristiaan Leeuwenburgh
Nov 19, 2015·Journal of Cellular Physiology·Jingjing FanNing Chen
Jan 1, 2012·Cells·Paolo Grumati, Paolo Bonaldo
Apr 8, 2018·Physiological Reports·Nina BrandtHenriette Pilegaard
Jan 23, 2016·The Journal of Experimental Biology·William J SmilesDonny M Camera
Oct 20, 2017·PloS One·Nina BrandtHenriette Pilegaard
Nov 16, 2018·Aging Cell·Kurt A EscobarTrisha A VanDusseldorp
Jun 16, 2012·American Journal of Physiology. Cell Physiology·Anthony M J SanchezHenri Bernardi
Sep 3, 2013·Physiology·Carmen Fiuza-LucesAlejandro Lucia
Nov 2, 2013·The Journal of Clinical Investigation·Md Shenuarin BhuiyanJeffrey Robbins
Aug 15, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Anthony M J SanchezRobin B Candau

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