Modulation of bacterial entry into epithelial cells by association between vinculin and the Shigella IpaA invasin

The EMBO Journal
G Tran Van NhieuP J Sansonetti

Abstract

Shigella flexneri is the causative agent of bacillary dysentery in humans. Shigella invasion of epithelial cells is characterized by cytoskeletal rearrangements and formation of cellular projections engulfing the bacterium in a macropinocytic process. We show here that vinculin, a protein involved in linking actin filaments to the plasma membrane, is a direct target of Shigella during cell invasion. IpaA, a Shigella protein secreted upon cell contact, rapidly associates with vinculin during bacterial invasion. Although defective for cell entry, an ipaA mutant is still able to induce foci of actin polymerization, but differs from wild-type Shigella in its ability to recruit vinculin and alpha-actinin. Presumably, IpaA-vinculin interaction initiates the formation of focal adhesion-like structures required for efficient invasion.

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Citations

Jan 1, 2013·Protoplasma·Joanne M Leerberg, Alpha S Yap
Nov 5, 1997·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·P J Sansonetti
Aug 21, 2003·Microbial Pathogenesis·Nurmohammad ShaikhHaruo Watanabe
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Feb 27, 1999·Current Opinion in Microbiology·G T Nhieu, P J Sansonetti
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Apr 16, 2002·Molecular Microbiology·Anne-Laure PageClaude Parsot
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Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·D ZhouJ E Galán
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Jan 7, 2009·Journal of Bacteriology·Kimberly A Walker, Virginia L Miller

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