Modulation of band 3-ankyrin interaction by protein 4.1. Functional implications in regulation of erythrocyte membrane mechanical properties.

The Journal of Biological Chemistry
X L AnNarla Mohandas

Abstract

Protein 4.1 is an important structural component of the erythrocyte membrane. In contrast to our detailed understanding of the role of protein 4.1 in regulating membrane mechanical properties through modulation of spectrin-actin interaction, very little is known regarding the functional implications of protein 4.1 interaction with band 3. In the present study, we explored the potential role of protein 4.1-band 3 interaction in modulating membrane mechanical properties. Based on recent studies which identified the sequence motif IRRRY in band 3 as the protein 4.1 interacting domain, we studied the functional consequences of specific dissociation of band 3-protein 4.1 interaction by the synthetic peptide IRRRY. We show that protein 4.1 bound to the inside-out vesicles could be dissociated from band 3 but not from glycophorin C by IRRRY. Furthermore, incorporation of IRRRY into resealed ghosts resulted in decreased membrane deformability and increased membrane mechanical stability. The observed alterations in membrane properties appears to result from increased band 3-ankyrin interaction following dissociation of protein 4.1 from band 3. These studies have enabled us to identify an important functional role for band 3-protein 4.1 ...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J M TylerD Branton
Nov 29, 1990·The New England Journal of Medicine·S C LiuP Jarolim
Mar 10, 1995·The Journal of Biological Chemistry·N J HemmingN Mohandas

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Citations

Feb 15, 2001·The Journal of Experimental Zoology·M W Musch, L Goldstein
Mar 28, 2003·Biochemical and Biophysical Research Communications·Yujuan ZhengJiazuan Ni
May 2, 2002·Clinical Biochemistry·Ludmila ZylinskaMiroslaw Soszynski
Jul 17, 1999·Brain Research. Molecular Brain Research·H YamakawaO Ohara
Aug 14, 2001·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J P Cartron, Y Colin
Aug 7, 1998·Molecular Biology of the Cell·T Tominaga, D L Barber
Aug 26, 1998·The EMBO Journal·T TominagaD L Barber
Sep 10, 2011·International Journal of Cell Biology·Wataru NunomuraYuichi Takakuwa
Jan 25, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nadezhda N BarvitenkoRoy E Weber
Jun 8, 1999·The Journal of Clinical Investigation·L L PetersC Brugnara
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sumie MannoNarla Mohandas
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·D C Ghislaine MayerLouis H Miller
Jun 23, 2009·Journal of Proteomics·Erica M PasiniAlan W Thomas
Dec 10, 2015·Frontiers in Physiology·Ying LiuLi-Ming Chen
Jun 16, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Wataru NunomuraKenji Murata
Jun 26, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kohei ShibaYuichi Takakuwa
Mar 14, 1998·Protein Expression and Purification·R F Workman, P S Low
Nov 6, 2012·Free Radical Biology & Medicine·Alessandro MatteLucia De Franceschi
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·L K PutneyD L Barber
Apr 8, 2017·Scientific Reports·Sandrine GenetetIsabelle Mouro-Chanteloup
Jun 18, 2005·Anatomical Science International·Nobuo TeradaShinichi Ohno
Aug 23, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nobuo TeradaShinichi Ohno
Apr 7, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Zhangli PengQiang Zhu
Jun 1, 2000·The Journal of Biological Chemistry·W NunomuraN Mohandas
Jun 10, 2006·The Journal of Biological Chemistry·William A AnongPhilip S Low
Dec 15, 2010·Expert Review of Proteomics·Susan YuditskayaGregory J Kato
Dec 25, 2010·Lab on a Chip·Pedro A Quinto-SuClaus-Dieter Ohl
Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Estela Puchulu-CampanellaPhilip S Low

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