Modulation of beta2- and beta3-adrenoceptor-mediated relaxation of rat oesophagus smooth muscle by protein kinase C

European Journal of Pharmacology
Jaap OostendorpJ Zaagsma

Abstract

Although a prominent role for protein kinase C (PKC) in the cross-talk between the phosphoinositide pathway and beta2-adrenoceptor signalling has been indicated, modulation of beta3-adrenoceptor function by PKC has not been studied thus far. In the present study, we have compared the relative capacity of PKC in modulating beta2- and beta3-adrenoceptor-mediated relaxation of methacholine-contracted rat oesophagus smooth muscle. To this purpose the effects of the PKC-inhibitor GF 109203X (2-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide) on relaxation induced by fenoterol, formoterol, (-)-noradrenaline, BRL 35135 (4-[2-[(2-hydroxy-2-(chlorophenyl)ethyl)amino]-propyl]-phenoxyacetic-acidmethylester) and IBMX (3-isobutyl-1-methyl-xanthine) were studied, in the absence and presence of the selective beta2-adrenoceptor antagonist ICI 118,551 (erythro-1(7-methylindan-4-yloxy)-3-(isopropylamin)-butan-2-ol). Our results show that inhibition of PKC resulted in differential augmentation of both beta2- and beta3-adrenoceptor-mediated relaxation. In contrast, relaxation induced by IBMX was not influenced at all by GF 109203X. The beta2-adrenoceptor bears phosphorylation sites for several kinases, including PKC. Since th...Continue Reading

References

Aug 4, 1995·The Journal of Biological Chemistry·T T ChuangA De Blasi
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S B LiggettR J Lefkowitz
Jan 1, 1997·Life Sciences·J ZaagsmaH Meurs
May 12, 1997·European Journal of Pharmacology·G RousseauM Bouvier
Nov 22, 1997·European Journal of Pharmacology·K KoikeT Nagatomo
Jan 9, 1999·European Journal of Pharmacology·G LenzenA D Strosberg
Mar 27, 2001·British Journal of Pharmacology·C M FilipeanuA Nelemans
Dec 4, 2002·European Journal of Pharmacology·Jaap OostendorpKnut Biber

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Citations

Jun 3, 2010·Naunyn-Schmiedeberg's Archives of Pharmacology·Martin C MichelRoger J Summers
May 26, 2005·European Journal of Pharmacology·Mark BotermanHerman Meurs
Sep 2, 2008·American Journal of Respiratory Cell and Molecular Biology·Caroline MorinEric Rousseau
Dec 14, 2007·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Kohsuke Uchida, Yuichiro Kamikawa
Feb 14, 2012·Canadian Journal of Physiology and Pharmacology·Caroline MorinEric Rousseau
Nov 13, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Lene H PetersenDuane B Huggett
May 10, 2017·American Journal of Obstetrics and Gynecology·Laurence MassenavetteÉric Rousseau
Jun 18, 2016·International Journal of Molecular Medicine·Hui-Wei HuangYi-Hua Qiu
Mar 5, 2011·BJU International·Martin HennenbergChristian Gratzke
Mar 7, 2021·Molecular Biology Reports·Yaqian Wang, Shujuan Jiang

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