Modulation of Biointeractions by Electrically Switchable Oligopeptide Surfaces: Structural Requirements and Mechanism

Advanced Materials Interfaces
Chun L YeungPaula M Mendes

Abstract

Understanding the dynamic behavior of switchable surfaces is of paramount importance for the development of controllable and tailor-made surface materials. Herein, electrically switchable mixed self-assembled monolayers based on oligopeptides have been investigated in order to elucidate their conformational mechanism and structural requirements for the regulation of biomolecular interactions between proteins and ligands appended to the end of surface tethered oligopeptides. The interaction of the neutravidin protein to a surface appended biotin ligand was chosen as a model system. All the considerable experimental data, taken together with detailed computational work, support a switching mechanism in which biomolecular interactions are controlled by conformational changes between fully extended ("ON" state) and collapsed ("OFF" state) oligopeptide conformer structures. In the fully extended conformation, the biotin appended to the oligopeptide is largely free from steric factors allowing it to efficiently bind to the neutravidin from solution. While under a collapsed conformation, the ligand presented at the surface is partially embedded in the second component of the mixed SAM, and thus sterically shielded and inaccessible for...Continue Reading

References

Jan 1, 1975·Advances in Protein Chemistry·N M Green
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·O LivnahJ L Sussman
Apr 23, 2003·Analytical Chemistry·Hidenori YamanakaTeruo Okano
Jul 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chunhai FanAlan J Heeger
Jul 19, 2003·Science·Dale L HuberBruce C Bunker
Sep 2, 2004·Journal of the American Chemical Society·Chad E ImmoosMark W Grinstaff
May 12, 2005·Journal of the American Chemical Society·Marco CurreliChongwu Zhou
Oct 20, 2005·Journal of the American Chemical Society·Subramanian BalamuruganMichael Grunze
Aug 9, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Kenichi NagaseTeruo Okano
Oct 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ulrich RantGerhard Abstreiter
Oct 27, 2007·Bioconjugate Chemistry·Paula M MendesJ Fraser Stoddart
Apr 12, 2008·Chembiochem : a European Journal of Chemical Biology·Douglas D Young, Alexander Deiters
Jun 24, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mark StevensonLeonard W Seymour
Jul 26, 2008·Nature Nanotechnology·Robert J MannixDonald E Ingber
Oct 25, 2008·Chemical Society Reviews·Paula M Mendes
Apr 5, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Yujin TongShen Ye
Dec 7, 2011·Molecular Pharmaceutics·Mahmoud SolimanCameron Alexander

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Citations

Jun 9, 2016·Accounts of Chemical Research·Eleonora CantiniPaula M Mendes
Mar 27, 2015·Advanced Materials Interfaces·Alice PranzettiPaula M Mendes
Mar 10, 2016·Chemical Communications : Chem Comm·Jugal Kishore SahooRein V Ulijn
Nov 2, 2014·Chemical Communications : Chem Comm·Minhaj LashkorPaula M Mendes
Jul 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Johanna BaumgartnerEdwin W H Jager
Feb 4, 2021·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Joshua S Gibson, Paula M Mendes
Jan 10, 2018·ACS Applied Materials & Interfaces·F DalierC Tribet

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Methods Mentioned

BETA
X-ray
surface plasmon resonance
chip

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