Modulation of bradykinin signaling by EP24.15 and EP24.16 in cultured trigeminal ganglia

Journal of Neurochemistry
Nathaniel A JeskeJames L Roberts

Abstract

Metalloendopeptidases expressed in neural tissue are characterized in terms of their neuropeptide substrates. One such neuropeptide, bradykinin (BK), is an important inflammatory mediator that activates the type-2 BK receptor (B2R) on the terminal endings of specialized pain-sensing neurons known as nociceptors. Among several metalloendopeptidases that metabolize and inactivate BK, EP24.15 and EP24.16 are known to associate with the plasma membrane in several immortalized cell lines. Potentially, the colocalization of EP24.15/16 and B2R at plasma membrane microdomains known as lipid rafts in a physiologically relevant nociceptive system would allow for discrete, peptidase regulation of BK signaling. Western blot analysis of crude subcellular fractions and lipid raft preparations of cultured rat trigeminal ganglia demonstrate similar expression profiles between EP24.15/16 and B2R on a subcellular level. Furthermore, the treatment of primary cultures of trigeminal ganglia with inhibitors of EP24.15/16 led to the potentiation of several bradykinin-induced events that occur downstream of B2R activation. EP24.15/16 inhibition by N-[1(R,S)-carboxy-3-phenylpropyl]-Ala-AlalTyr-p-aminobenzoate (cFP) resulted in a 1000-fold increase in B...Continue Reading

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Nov 5, 2003·Journal of Neuroscience Research·Nathaniel A JeskeJames L Roberts

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Citations

Jul 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Amol M PatwardhanKenneth M Hargreaves
Apr 11, 2014·The Journal of Biological Chemistry·Diogo M L P CavalcantiEmer S Ferro
Apr 24, 2008·The Journal of Pharmacology and Experimental Therapeutics·Caroline SandénL M Fredrik Leeb-Lundberg
Nov 16, 2014·The Journal of Physiology·Xin JinNikita Gamper
Mar 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mayara C F GewehrEmer S Ferro
Apr 30, 2020·Science Signaling·Shihab ShahNikita Gamper
Sep 21, 2007·Current Eye Research·Rubens Bertazolli-FilhoEduardo Miguel Laicine
Aug 8, 2020·Stem Cells Translational Medicine·Sara Mirali, Aaron D Schimmer

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