Modulation of bullous pemphigoid antigens by gamma interferon in cultured human keratinocytes

The Journal of Dermatology
H SuJ Thivolet

Abstract

We report the effects of human recombinant gamma interferon (gamma-IFN) on the expression of bullous pemphigoid (BP) antigens by human cultured keratinocytes. Secondary epidermal cell cultures were grown on 3T3 mouse fibroblasts; when confluent, some cultures were maintained in control medium while others were exposed to various concentrations of gamma-IFN (100, 200, 400 U/ml) for 14 days. The expression of BP antigens was analyzed by indirect immunofluorescence on epithelial sheets and immunoblotting of Tris, SDS, beta-mercaptoethanol culture extracts using different BP sera. Our results show that gamma-IFN alters the expression of BP antigens in a way varying according to the skin donor: we observed results ranging from complete loss and decreased expression to unchanged reactivity patterns. Thus, gamma-IFN modifies BP antigen expression; this behavior has been previously shown for other adhesion molecules such as fibronectin and thrombospondin. However, the observed variability of the expression of BP antigens according to the skin donor suggests an unexpected variability in keratinocyte sensitivity to gamma interferon, which remains to be explored both in vitro and in vivo.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H GreenJ Thomas
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1990·Archives of Dermatological Research·A ReanoJ Viac
Jan 1, 1986·Ultrastructural Pathology·B J NickoloffV Morhenn
May 1, 1987·The Journal of Investigative Dermatology·J N MansbridgeV B Morhenn
Aug 1, 1987·The Journal of Investigative Dermatology·B J Nickoloff
Aug 1, 1988·The British Journal of Dermatology·B Volc-PlatzerK Wolff
Dec 1, 1988·The Journal of Clinical Investigation·J R StanleyD Roop
Dec 1, 1988·Immunology Today·J A Langer, S Pestka
Jan 1, 1988·Archives of Dermatological Research·G Hamm, K D Wozniak
Nov 1, 1986·The Journal of Investigative Dermatology·N E WiknerW L Weston
Sep 1, 1985·The Journal of Investigative Dermatology·M RegnierM Prunieras
Jan 1, 1985·Archives of Dermatological Research·J AuböckC Huber
Jul 1, 1985·The Journal of Investigative Dermatology·V B MorhennJ N Mansbridge
Feb 1, 1984·Journal of Clinical Pathology·P G Higgins
Apr 1, 1984·The Journal of Investigative Dermatology·C H ThivoletJ R Stanley
Apr 1, 1982·Journal of the American Academy of Dermatology·W M Sams, W R Gammon

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Citations

Jun 27, 2008·The Journal of Investigative Dermatology·Soheil S DadrasMichael Detmar
Nov 28, 2017·Journal of the European Academy of Dermatology and Venereology : JEADV·A Abdelmaksoud
Jun 1, 1993·The Journal of Investigative Dermatology·A ReanoD Schmitt

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