Modulation of Ca2+ channel currents of acutely dissociated rat periaqueductal grey neurons

The Journal of Physiology
M Connor, M J Christie

Abstract

1. The actions of the neuropeptide nociceptin on the calcium channel currents (IBa) of acutely dissociated rat periaqueductal grey (PAG) neurons were examined using whole-cell patch clamp techniques. These effects were compared with those of opioid receptor agonists and the GABAB receptor agonist baclofen. 2. Neurons from young adult rats (23 to 56 days old) expressed predominantly omega-conotoxin GVIA (N-type)- and omega-agatoxin IVA (P/Q-type)-sensitive IBa, together with smaller amounts of nimodipine-sensitive current and current resistant to all three blockers. There was proportionately more N-type IBa in neurons from female rats and proportionately more resistant current in neurons from male rats. 3. Nociceptin (EC50, 5 nM) and baclofen (EC50, 0.8 microM) inhibited IBa in all PAG neurons, while the opioid agonist methionine enkephalin (met-enkephalin; 300 nM-10 microM) inhibited IBa in 40 % of neurons. The effects of met-enkephalin were reversed by the mu-opioid antagonist CTAP, and mimicked by the mu-opioid agonist DAMGO (300 nM-3 microM). The delta-opioid agonists DPDPE and deltorphin II, and the kappa-opioid agonist U69593, did not affect IBa in any neuron. The actions of nociceptin were not mimicked or blocked by the o...Continue Reading

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Citations

Feb 27, 2003·Biochemical and Biophysical Research Communications·Kun YangMegumu Yoshimura
Sep 22, 2000·Peptides·T D MoranP A Smith
Jun 30, 2000·Clinical and Experimental Pharmacology & Physiology·M J ChristieE E Bagley
Nov 6, 2009·The Journal of Biological Chemistry·Rhian M EvansGerald W Zamponi
Sep 6, 2008·Cephalalgia : an International Journal of Headache·G MartellaP Calabresi
May 29, 2012·Pain Practice : the Official Journal of World Institute of Pain·George MiljanichMichael Saulino
Oct 7, 2004·European Journal of Pharmacology·Ryszard Przewlocki
Apr 24, 2003·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Susanne Meis
Apr 7, 2017·Scientific Reports·Salvatore PacificoRemo Guerrini
Jan 20, 2004·Nature Neuroscience·Aaron M BeedleGerald W Zamponi
Nov 29, 2005·Nature Neuroscience·Christophe AltierGerald W Zamponi
Sep 22, 1999·European Journal of Biochemistry·J I FletcherG F King
Jun 23, 2004·Nature Reviews. Neuroscience·Howard Fields
Jul 22, 2004·British Journal of Pharmacology·Mark ConnorChristopher W Vaughan
Nov 30, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Susan L IngramMichael M Morgan
May 3, 2003·Nature Reviews. Neuroscience·Daniela Pietrobon, Jörg Striessnig
May 17, 2003·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Jean-Claude Meunier
Nov 13, 2010·Cardiovascular Therapeutics·William M Armstead

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