Modulation of Ca2+ sensitivity regulates contractility of rabbit corpus cavernosum smooth muscle

The Journal of Urology
Ryosuke TakahashiHideo Kanaide

Abstract

We examined the role of the modulation of Ca2+ sensitivity for regulating the contractility of corpus cavernosum smooth muscle. We applied simultaneous measurements of intracellular Ca2+ concentration and tension in fura-PE3 loaded intact strips and receptor coupled permeabilization by alpha-toxin. In intact fura-PE3 loaded strips the tension induced by 10 microM. phenylephrine was significantly greater than that produced by depolarization with 118 mM. K+, although the extent of intracellular Ca2+ concentration elevations was similar. During sustained contraction induced by 10 microM. phenylephrine the application of 10 microM. Y-27632 (a Rho kinase inhibitor) induced relaxation with a slight decrease in intracellular Ca2+ concentration, while the application of 3 microM. GF109203X (a protein kinase C inhibitor) induced relaxation without changing intracellular Ca2+ concentration. In alpha-toxin permeabilized strips 10 microM. phenylephrine induced a larger increase in force at a constant intracellular Ca2+ concentration and produced a leftward shift in the intracellular Ca2+ concentration-tension relationship, a response that was partially inhibited by pretreatment with Y-27632 or GF109203X. These results indicate that in rabb...Continue Reading

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Citations

Oct 21, 2006·Current Urology Reports·Trinity J Bivalacqua, Arthur L Burnett
Jul 19, 2011·Asian Journal of Andrology·Otacilio D BenvindoVietla S Rao
Oct 7, 2011·International Journal of Impotence Research·X Jiang, K Chitaley
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Nov 23, 2006·European Urology·Trinity J BivalacquaArthur L Burnett
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Oct 30, 2016·Basic & Clinical Pharmacology & Toxicology·Martin GrannUlf Simonsen
Sep 21, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xueqin Ding, Paul A Murray
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Jan 18, 2006·Clinical Science·Liming Jin, Arthur L Burnett
Jan 30, 2021·Biology of Reproduction·Daiki HashimotoGen Yamada

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