Modulation of Ca2+ transients and tension by intracellular EGTA in intact frog muscle fibers

The American Journal of Physiology
J D JohnsonM Flynn

Abstract

Ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) was used as an intracellular Ca2+ chelator to shorten the duration of the Ca2+ transient and to determine the rate-limiting steps in relaxation of frog skeletal muscle at 10 and 20 degrees C. Incubation with the acetoxymethyl ester of EGTA (EGTA-AM) produced a linear approximately twofold increase in the rate of fall in twitch Ca2+ concentration ([Ca2+]) over 0-70 min at 10 degrees C. The rate of relaxation initially increased from 5 to 9/s over 0-13 min and then leveled, as the rate of fall in [Ca2+] continued to increase and peak force decreased. Increasing the rate of fall in [Ca2+] increased the rate of relaxation, until a rate-limiting step was reached at approximately 9/s at 10 degrees C. At 20 degrees C, incubation with EGTA-AM produced a linear approximately twofold increase in the rate of fall in twitch [Ca2+] without affecting the rate of relaxation (27/s). Ca2+ dissociated from the regulatory sites of purified troponin (Tn) at approximately 8/s at 10 degrees C and at 23/s at 20 degrees C. When the duration of the Ca2+ transient was decreased by EGTA or increased by partial inhibition of the sarcoplasmic reticulum Ca(2+)-ATPase, twitch force ...Continue Reading

References

Aug 1, 1992·Journal of Muscle Research and Cell Motility·Z GrabarekJ Gergely
Jan 1, 1990·Annual Review of Physiology·E Homsher, N C Millar
Dec 1, 1982·Journal of Muscle Research and Cell Motility·J M GillisR H Kretsinger
Jun 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S Farah, F C Reinach

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Citations

Nov 28, 2000·Cell Motility and the Cytoskeleton·B W BernsteinJ R Bamburg
Sep 24, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Jonathan F McAnultyChristopher J Murphy
Jul 1, 2005·Pflügers Archiv : European journal of physiology·Gias U Ahmmed, Asrar B Malik
Jan 23, 2015·Pulmonary Circulation·Sukriti SukritiDolly Mehta
Sep 23, 2014·Journal of Muscle Research and Cell Motility·Juan C CalderónCarlo Caputo
Apr 1, 2017·Military Medical Research·Chen-Yang DuanLiang-Ming Liu
Apr 22, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Hengrui SunMack H Wu
Aug 16, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Max I Bogorad, Peter C Searson
Aug 7, 2001·Journal of Endodontics·A M Cruz-FilhoJ D Pécora
Jul 27, 2000·Endothelium : Journal of Endothelial Cell Research·L M Defouw, D O Defouw
Feb 28, 2009·Stroke; a Journal of Cerebral Circulation·Christoph R W KuhlmannHeiko J Luhmann
Dec 3, 2016·Thrombosis and Haemostasis·Dursun GündüzMuhammad Aslam
Nov 15, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Peng YuWalter N Durán
Nov 26, 2016·Physiological Reports·Ian C SmithA Russell Tupling
Sep 19, 2015·Scientific Reports·Weijin ZhangXiaohua Guo
Jun 5, 1998·The American Journal of Physiology·P A WahrJ A Rall
Dec 8, 2007·Journal of Cellular Physiology·Anna A BirukovaKonstantin G Birukov

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