PMID: 8584434Jan 1, 1996Paper

Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells

Pflügers Archiv : European journal of physiology
K OtsuguroY Nakazato

Abstract

The effects of extracellular adenosine 5'-triphosphate (ATP) on voltage-dependent Ca2+ currents were examined using the whole-cell voltage-clamp technique in guinea-pig isolated adrenal chromaffin cells. ATP (500 microM) reversibly suppressed Ca2+ currents in the presence of 5mM Ca2+ in the extracellular solution. The inhibitory effect of ATP on Ca2+ currents tended to increase with increases in the peak amplitude of ATP-evoked current when the intracellular solution contained 0.1 or 1 mM ethylenebis(oxonitrilo)tetraacetate(EGTA). Using the intracellular solution containing 10mM EGTA, on the other hand, the inhibitory effect did not change regardless of the amplitude of current responses to ATP. In the presence of 10 mM Ba2+, ATP (100 microM) reduced Ba2+ currents in a manner similar to Ca2+ currents. This reduction was decreased by dialysis of cells with the internal solution containing guanosine 5'-O-(2-thiodiphosphate) (GDP [beta-S]; 1 mM) or guanosine 5'-O-(3-thiotriphosphate) (GTP [gamma-S]; 100 microM). A depolarizing prepulse to + 100 mV partly relieved ATP-induced reduction of Ba2+ currents. ADP, AMP and adenosine also reduced Ba2+ currents and the effect of adenosine was the most potent. Adenosine (0.5 and 1 mM) signif...Continue Reading

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Citations

Jun 5, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Y KasaiY Nakazato
Aug 24, 1999·The European Journal of Neuroscience·E A Lukyanetz, E Neher
Sep 6, 2005·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Mekbeb Afework, Geoffrey Burnstock
Jun 6, 1998·The Journal of Physiology·S KobayashiD E Millhorn
May 11, 2001·The Journal of Veterinary Medical Science·Y KasaiS Ito

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