Modulation of Candida albicans virulence by bacterial biofilms on titanium surfaces

Biofouling
Yuri Wanderley CavalcantiDavid Wynne Williams

Abstract

Whilst Candida albicans occurs in peri-implant biofilms, its role in peri-implantitis remains unclear. This study therefore examined the virulence of C. albicans in mixed-species biofilms on titanium surfaces. Biofilms of C. albicans (Ca), C. albicans with streptococci (Streptococcus sanguinis, S. mutans) (Ca-Ss-Sm) and those incorporating Porphyromonas gingivalis (Ca-Pg and Ca-Ss-Sm-Pg) were developed. Expression of C. albicans genes associated with adhesion (ALS1, ALS3, HWP1) and hydrolytic enzymes (SAP2, SAP4, SAP6, PLD1) was measured and hyphal production by C. albicans quantified. Compared with Ca biofilms, significant (p<0.05) up-regulation of ALS3, HWP1, SAP2 and SAP6, and hyphal production occurred in biofilms containing streptococci (Ca-Ss-Sm). In Ca-Pg biofilms, down-regulation of HWP1 and SAP4 expression, with reduced hyphal production occurred. Ca-Ss-Sm-Pg biofilms had increased hyphal proportions and up-regulation of ALS3, SAP2 and SAP6. In conclusion, C. albicans expressed virulence factors in biofilms that could contribute to peri-implantitis, but this was dependent on associated bacterial species.

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Citations

Nov 1, 2016·Acta Odontologica Scandinavica·Carla Paola Lozano MoragaBlanca Regina Urzúa Orellana
Jun 13, 2017·Journal of Dental Research·K EllepolaC J Seneviratne
Jan 24, 2019·International Journal of Environmental Research and Public Health·Humidah AlanaziMahmoud Rouabhia
Aug 14, 2019·Periodontology 2000·Simone CortelliniMarc Quirynen
Sep 17, 2019·Journal of Medical Microbiology·Jason L BrownGordon Ramage
Mar 16, 2019·Medical Mycology·Laura Judith Marcos-ZambranoJesús Guinea
Feb 12, 2020·The ISME Journal·João Gabriel Silva SouzaAnna Dongari-Bagtzoglou
Nov 8, 2018·Journal of Fungi·Daniel Montelongo-Jauregui, Jose L Lopez-Ribot
Mar 1, 2020·Applied and Environmental Microbiology·João Gabriel Silva SouzaAnna Dongari-Bagtzoglou
Apr 5, 2020·BMC Complementary Medicine and Therapies·Loyse Martorano-FernandesLeopoldina de Fátima Dantas de Almeida
Dec 3, 2020·BMC Oral Health·Loyse Martorano-FernandesLeopoldina de Fátima Dantas de Almeida
Jun 18, 2021·International Journal of Implant Dentistry·Irene Lafuente-Ibáñez de MendozaJosé Manuel Aguirre-Urizar
Aug 28, 2021·Clinical Oral Investigations·Isabelle Helena Gurgel de CarvalhoRodrigo Othávio de Assunção E Souza

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Methods Mentioned

BETA
reverse transcription PCR
electrophoresis
PCR

Software Mentioned

SPSS for
Primer3
ImageJ
analyse particles

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