Modulation of catecholamine-synthesizing enzymes in the rat heart by repeated immobilization stress

Annals of the New York Academy of Sciences
L MicutkovaR Kvetnanský

Abstract

Stress is one of the major risk factors responsible for the increased incidence of a number of common life-threatening disorders, predominantly of cardiovascular origin. The aim of the present study was to establish the effect of repeated immobilization stress on gene expression and protein levels of aromatic L-amino acid decarboxylase (AADC) and phenylethanolamine N-methyltransferase (PNMT) in cardiac left and right atria. In the process of repeated immobilization, rats were immobilized 2 h daily for 7 days and killed 3 h after the last immobilization. Gene expression was determined by regular and real-time reverse transcription with subsequent polymerase chain reaction, and protein levels were determined by Western blot analysis. In cardiac atria, we identified mRNA for AADC and PNMT. Repeated immobilization stress did not affect AADC mRNA levels. However, repeated immobilization significantly increased PNMT mRNA levels compared with unstressed control animals. No further increase was observed compared with adapted control rats (rats immobilized six times for 2 h daily and decapitated 24 h after the sixth immobilization). AADC protein levels corresponded with mRNA levels of this enzyme. However, we were not able to detect PNM...Continue Reading

Citations

Jan 29, 2011·Current Hypertension Reports·Elisabeth A Lambert, Gavin W Lambert
Jun 12, 2009·Journal of Hypertension·Murray Esler
Mar 1, 2008·Clinical and Experimental Pharmacology & Physiology·Murray EslerElisabeth Lambert
Jul 9, 2004·Annals of the New York Academy of Sciences·Richard KvetnanskyOlga Krizanova
Jan 6, 2009·Annals of the New York Academy of Sciences·Murray EslerElisabeth Lambert
May 27, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Toshio Obata
Dec 17, 2011·Acta Physiologica Hungarica·N SpasojevicSladjana Dronjak
Nov 7, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L GavrilovicS Dronjak
May 22, 2007·Stress : the International Journal on the Biology of Stress·O KrizanovaL Kubovcakova
Apr 4, 2009·Physiological Reviews·Richard KvetnanskyMiklos Palkovits
Feb 8, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Grazia ChielliniRiccardo Zucchi
Jan 13, 2021·Clinical Autonomic Research : Official Journal of the Clinical Autonomic Research Society·Murray Esler

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