Modulation of cell-associated plasminogen activator activity by cocultivation of a stem cell and its tumorigenic descendant.

Molecular and Cellular Biology
H Y LiuW F Mangel

Abstract

The effect of the presence of one cell type on the plasminogen activator activity of another cell type was studied. The cell types, AC and D, were isolated from a rat neuroblastoma (I. Imada and N. Sueoka, Dev. Biol. 66:97-108, 1978). AC cells are stem cells capable of multipotential differentiation in vitro and have little or no cell-associated plasminogen activator activity. D cells are tumorigenic and have high levels of cell-associated plasminogen activator activity. When AC cells were cocultivated with D cells, the plasminogen activator activity of the D cells was dramatically inhibited. The presence of as few as 1,250 AC cells inhibited 70% of the plasminogen activator activity of 20,000 D cells, as determined by a highly quantitative assay. The amount of inhibition by AC cells was proportional to the number of AC cells present. At increasing numbers of AC cells and a constant number of D cells, the Vmax for the activation of plasminogen proportionately decreased and the Km remained constant, implying that AC cells did not alter the structure or concentration of plasminogen. Inhibition was not mediated by a soluble inhibitor secreted by AC cells. Rather, attachment of AC cells adjacent to D cells, i.e., cell-to-cell conta...Continue Reading

References

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Nov 1, 1982·Molecular and Cellular Biology·H Y LiuW F Mangel

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Citations

Jun 25, 1984·FEBS Letters·E M SalonenA Vaheri
Mar 6, 2009·Nuclear Medicine Communications·Ali GholamrezanezhadReza Malekzadeh
Nov 12, 1985·Biochimica Et Biophysica Acta·O Saksela

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