PMID: 9185338May 1, 1997Paper

Modulation of chloride secretion in the rat ileum by intracellular bicarbonate

Comparative Biochemistry and Physiology. Part A, Physiology
P C DagherA N Charney

Abstract

Increasing intracellular bicarbonate concentration ([HCO3-]i) inhibits calcium-mediated Cl- secretion in rat distal colon and T84 cells. We investigated the effect of [HCO3-]i on Cl- secretion in rat ileum. Segments of intact ileum from Sprague-Dawley rats were studied in Ussing chambers and villus and crypt intracellular pH and [HCO3-]i were determined using BCECF. A range of crypt and villus [HCO3-]i from 0 to 31 mM was obtained by varying Ringer's composition. Basal serosal-to-mucosal Cl- flux (JsmCl) averaged 8.5 +/- 0.2 mu eq.h-1.cm-2 and was unaffected by changing [HCO3-]i or serosal bumetanide. Carbachol increased JsmCl by 3.9 +/- 0.5 mu eq.h-1.cm-2 at [HCO3-]i = 0 mM but only by 1.0 +/- 0.3 mu eq.h-1.cm-2 at high crypt and villus [HCO3-]i. Dibutyryl-cAMP increased JsmCl by 2.5 +/- 0.2 mu eq.h-1.cm-2 at all [HCO3-]i. Carbachol and db-cAMP showed mutual antagonism at low [HCO3-]i and near-additivity at high [HCO3-]i. We conclude that like rat colon and T84 cells, calcium-mediated but not cAMP-mediated Cl- secretion in the ileum is inhibited by increasing [HCO3-]i. Mutual antagonism between carbachol and db-cAMP at low [HCO3-]i was present in ileum and distal colon but not in T84 cells.

References

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Citations

Sep 2, 2000·Journal of Biochemical and Molecular Toxicology·M A El-Missiry, F Shalaby
Mar 18, 2004·Biochimica Et Biophysica Acta·Alan N CharneyMitchell B Cohen
Sep 27, 2003·Journal of Inorganic Biochemistry·Daniel J DortaCarlos Curti
Jan 12, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·A N CharneyR W Egnor
Apr 22, 2010·The Journal of Clinical Investigation·Kuai YuH Criss Hartzell
Aug 17, 2004·The Journal of Physiology·Zoltan SuttoMatthias Salathe
Apr 13, 2004·American Journal of Physiology. Cell Physiology·Alan N CharneyGurdip S Sidhu

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