PMID: 9444976Aug 1, 1997Paper

Modulation of class I major histocompatibility complex antigen cell-surface stability by transmembrane domain length variation

Molecular Immunology
C K OsbornM D Crew

Abstract

Cell surface localization of major histocompatibility complex (MHC) class I proteins is conferred to a large extent by their transmembrane domains (TMs) which exhibit allelic, inter-locus and inter-species variation in both amino acid composition and length. Here, the consequences of TM length variation on trafficking and cell-surface stability were examined using the human MHC class I protein HLA-A2. Transformed B lymphocytes (CIR cells) transfected with an HLA-A2 gene encoding an additional 12 hydrophobic amino acids in the TM exhibited a marked decrease in steady-state cell-surface levels of the HLA-A2 protein relative to cells transfected with the wild-type HLA-A2 gene. Diminished surface expression was observed regardless of the presence or absence of the cytoplasmic domain and could not be accounted for by altered association with beta2-microglobulin. While intracellular trafficking rates were affected by TM length enlargement and/or the absence of cytoplasmic domains, this, as well, could not completely explain the TM length-dependent differential cell-surface levels. Studies using brefeldin A to block transport of HLA-A2 proteins to the cell surface suggested that the diminished cell-surface levels of TM enlarged HLA-A2...Continue Reading

References

Feb 1, 1992·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·J Trowsdale, R D Campbell
Jul 27, 1990·Cell·J W Yewdell, J R Bennink
Jul 1, 1991·Human Immunology·J Zemmour, P Parham
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M D CrewR L Walford
Aug 2, 1990·Nature·H G LjunggrenK Kärre
Jan 1, 1990·Immunogenetics·M D CrewR L Walford
Dec 1, 1989·The Journal of Experimental Medicine·K A BrorsonL Hood
Jan 1, 1989·Immunogenetics·J AlexanderP Cresswell
Jan 1, 1993·Annual Review of Immunology·H G RammenseeO Rötzschke
Jan 1, 1993·Annual Review of Immunology·R N Germain, D H Margulies
Jun 1, 1995·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·S A EllisW I Morrison
May 1, 1996·Analytical Biochemistry·V G GrigorievM D Crew
Jan 1, 1997·Immunogenetics·S GaudieriR L Dawkins

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Citations

Jun 7, 2005·Human Immunology·Wendy T N SwelsenElla M van den Berg-Loonen
Mar 21, 2003·Biophysical Chemistry·Raphael ZidovetzkiIsrael Pecht
Nov 26, 2002·Tissue Antigens·W T N SwelsenE M van den Berg-Loonen
Mar 3, 2004·Tissue Antigens·W T N SwelsenE M van den Berg-Loonen
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xuefei ShenRobert F Siliciano

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