PMID: 8600089Mar 15, 1996Paper

Modulation of clonogenicity, growth, and radiosensitivity of three human epidermoid tumor cell lines by a fibroblastic environment

International Journal of Radiation Oncology, Biology, Physics
B GeryJ B Little

Abstract

To develop a model vitro system to examine the influence of fibroblasts on the growth and survival of human tumor cells after exposure to ionizing radiation. The cell system of three epidermoid carcinoma cell lines derived from head and neck tumors having differing growth potentials and intrinsic radiosensitivities, as well as a low passage skin fibroblast strain from a normal human donor. The tumor cells were seeded for five days prior to exposure to radiation: (a) in the presence of different numbers of fibroblasts, (b) in conditioned medium from stationary fibroblast cultures, and (c) on an extracted fibroblastic matrix. When grown with fibroblasts, all three tumor cell lines showed increased clonogenicity and increased radioresistance. The radioprotective effect was maximal at a density of approximately 10(5) fibroblasts/100 mm Petri dish, and was greatest in the intrinsically radiosensitive tumor cell line. On the other hand, the effects of incubation with conditioned medium or on a fibroblastic matrix varied among the tumor cell lines. Thus, the protective effect afforded by coculture with fibroblasts must involve several cellular factors related to the fibroblast itself. These observations emphasize the importance of cul...Continue Reading

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Citations

Apr 24, 2001·International Journal of Radiation Oncology, Biology, Physics·J KummermehrK R Trott
May 14, 2008·International Journal of Radiation Oncology, Biology, Physics·Yuka NakazawaShunichi Yamashita
Aug 1, 2007·Free Radical Biology & Medicine·Clara BiondaDominique Ardail
Feb 18, 2014·International Journal of Radiation Biology·Sudhir ChandnaSiamak Haghdoost
Mar 4, 2000·International Journal of Cancer. Journal International Du Cancer·L RossiR Corvó

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